Morphological and molecular description of a new species of (Myxosporea: Myxobolidae) infecting (Smith, 1846) from India.

The present paper describes a novel species of parasitizing the gill filaments of the largescale mullet, from Cochin backwaters, Kerala, India. The parasite develops in the gill filaments; plasmodia elongated, milky white, measured 1.37-2.18 (1.78 ± 0.35) mm × 0.07-0.12 (0.10 ± 0.02) mm in size. Mature myxospores ovoid in valvular view, biconvex in sutural view with smooth shell valves and measured 6.24-7.02 (6.63 ± 0.23) × 5.01-6.18 (5.68 ± 0.25) μm in size. Polar capsules equal, oval with pointed anterior ends, 3.07-3.58 (3.33 ± 0.12) × 1.68-2.42 (2.09 ± 0.18) μm in size. Polar filaments with 4 coils, measured 29.61 ± 4.75 μm in length when extruded. Sporoplasm binucleate with a rudimentary nucleus and a vacuole. A comparison with related species revealed significant morphological and morphometric differences. In BLASTN and genetic distance analysis, the present parasite showed high divergence with other myxosporean sequences, indicating its molecular uniqueness. In Maximum Likelihood and Bayesian Inference analysis, the present species stands out with as sister branch within the clade. In infected gill filaments, the plasmodia caused swelling/deformation, compression of lamellae and reduction in respiratory surface area. Three of 222 screened were infected, indicating a prevalence of 1.3%. Considering the morphological, morphometric, molecular and phylogenetic differences with the previously described species of myxosporeans, along with the dissimilarities in host and geographical locations, the present parasite is treated as a new species and the name n. sp. is proposed.