• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

微小RNA-30a调控CAGE和p53的表达并调节对抗癌药物的反应。

miR-30a Regulates the Expression of CAGE and p53 and Regulates the Response to Anti-Cancer Drugs.

作者信息

Park Deokbum, Kim Hyuna, Kim Youngmi, Jeoung Dooil

机构信息

Department of Biochemistry, College of Natural Sciences, Kangwon National University, Chunchon 200-701, Korea.

出版信息

Mol Cells. 2016 Apr 30;39(4):299-309. doi: 10.14348/molcells.2016.2242. Epub 2016 Feb 25.

DOI:10.14348/molcells.2016.2242
PMID:26912082
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4844936/
Abstract

We have previously reported the role of miR-217 in anti-cancer drug-resistance. miRNA array and miRNA hybridization analysis predicted miR-30a-3p as a target of miR-217. miR-30a-3p and miR-217 formed a negative feedback loop and regulated the expression of each other. Ago1 immunoprecipitation and co-localization analysis revealed a possible interaction between miR-30a-3p and miR-217. miR-30a-3p conferred resistance to anti-cancer drugs and enhanced the invasion, migration, angiogenic, tumorigenic, and metastatic potential of cancer cells in CAGE-dependent manner. CAGE increased the expression of miR-30a-3p by binding to the promoter sequences of miR-30a-3p, suggesting a positive feedback loop between CAGE and miR-30a-3p. miR-30a-3p decreased the expression of p53, which showed the binding to the promoter sequences of miR-30a-3p and CAGE in anti-cancer drug-sensitive cancer cells. Luciferase activity assays showed that p53 serves as a target of miR-30a. Thus, the miR-30a-3p-CAGE-p53 feedback loop serves as a target for overcoming resistance to anti-cancer drugs.

摘要

我们之前报道了miR-217在抗癌药物耐药性中的作用。miRNA阵列和miRNA杂交分析预测miR-30a-3p是miR-217的一个靶点。miR-30a-3p和miR-217形成了一个负反馈环并相互调节表达。AGO1免疫沉淀和共定位分析揭示了miR-30a-3p与miR-217之间可能存在的相互作用。miR-30a-3p赋予癌细胞对抗癌药物的耐药性，并以依赖CAGE的方式增强癌细胞的侵袭、迁移、血管生成、致瘤和转移潜能。CAGE通过与miR-30a-3p的启动子序列结合来增加miR-30a-3p的表达，这表明CAGE与miR-30a-3p之间存在正反馈环。miR-30a-3p降低了p53的表达，在抗癌药物敏感的癌细胞中，p53显示出与miR-30a-3p和CAGE的启动子序列结合。荧光素酶活性测定表明p53是miR-30a的一个靶点。因此，miR-30a-3p-CAGE-p53反馈环可作为克服抗癌药物耐药性的一个靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cc8/4844936/d08c6e75577f/molce-39-4-299f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cc8/4844936/6eaa04223645/molce-39-4-299f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cc8/4844936/1949ccd33a56/molce-39-4-299f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cc8/4844936/e8ff26448735/molce-39-4-299f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cc8/4844936/b3d94eecef61/molce-39-4-299f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cc8/4844936/6300f65a6ed5/molce-39-4-299f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cc8/4844936/23db30eafa03/molce-39-4-299f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cc8/4844936/d08c6e75577f/molce-39-4-299f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cc8/4844936/6eaa04223645/molce-39-4-299f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cc8/4844936/1949ccd33a56/molce-39-4-299f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cc8/4844936/e8ff26448735/molce-39-4-299f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cc8/4844936/b3d94eecef61/molce-39-4-299f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cc8/4844936/6300f65a6ed5/molce-39-4-299f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cc8/4844936/23db30eafa03/molce-39-4-299f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cc8/4844936/d08c6e75577f/molce-39-4-299f7.jpg

相似文献

1
miR-30a Regulates the Expression of CAGE and p53 and Regulates the Response to Anti-Cancer Drugs.微小RNA-30a调控CAGE和p53的表达并调节对抗癌药物的反应。
Mol Cells. 2016 Apr 30;39(4):299-309. doi: 10.14348/molcells.2016.2242. Epub 2016 Feb 25.
2
miR-217 and CAGE form feedback loop and regulates the response to anti-cancer drugs through EGFR and HER2.微小RNA-217与癌症相关基因（CAGE）形成反馈回路，并通过表皮生长因子受体（EGFR）和人表皮生长因子受体2（HER2）调节对抗癌药物的反应。
Oncotarget. 2016 Mar 1;7(9):10297-321. doi: 10.18632/oncotarget.7185.
3
miR-200b and cancer/testis antigen CAGE form a feedback loop to regulate the invasion and tumorigenic and angiogenic responses of a cancer cell line to microtubule-targeting drugs.miR-200b 和癌症/睾丸抗原 CAGE 形成反馈回路，调节癌细胞系对微管靶向药物的侵袭、致瘤和血管生成反应。
J Biol Chem. 2013 Dec 20;288(51):36502-18. doi: 10.1074/jbc.M113.502047. Epub 2013 Oct 30.
4
Histone Deacetylase-3/CAGE Axis Targets EGFR Signaling and Regulates the Response to Anti-Cancer Drugs.组蛋白去乙酰化酶-3/CAGE轴靶向表皮生长因子受体信号传导并调节对抗癌药物的反应。
Mol Cells. 2016 Mar;39(3):229-41. doi: 10.14348/molcells.2016.2244. Epub 2016 Feb 16.
5
miR-326-histone deacetylase-3 feedback loop regulates the invasion and tumorigenic and angiogenic response to anti-cancer drugs.微小RNA-326-组蛋白去乙酰化酶-3反馈环调节对抗癌药物的侵袭、致瘤及血管生成反应。
J Biol Chem. 2014 Oct 3;289(40):28019-39. doi: 10.1074/jbc.M114.578229. Epub 2014 Aug 19.
6
Cancer/testis antigen CAGE exerts negative regulation on p53 expression through HDAC2 and confers resistance to anti-cancer drugs.癌/睾丸抗原 CAGE 通过 HDAC2 对 p53 表达发挥负调控作用，并赋予对癌症药物的耐药性。
J Biol Chem. 2010 Aug 20;285(34):25957-68. doi: 10.1074/jbc.M109.095950. Epub 2010 Jun 8.
7
p53/miR-30a-5p/ SOX4 feedback loop mediates cellular proliferation, apoptosis, and migration of non-small-cell lung cancer.p53/miR-30a-5p/SOX4 反馈环介导非小细胞肺癌细胞的增殖、凋亡和迁移。
J Cell Physiol. 2019 Dec;234(12):22884-22895. doi: 10.1002/jcp.28851. Epub 2019 May 23.
8
miRomics and Proteomics Reveal a miR-296-3p/PRKCA/FAK/Ras/c-Myc Feedback Loop Modulated by HDGF/DDX5/β-catenin Complex in Lung Adenocarcinoma.miRomics 和蛋白质组学揭示了 miR-296-3p/PRKCA/FAK/Ras/c-Myc 反馈环受肺腺癌中 HDGF/DDX5/β-catenin 复合物调控。
Clin Cancer Res. 2017 Oct 15;23(20):6336-6350. doi: 10.1158/1078-0432.CCR-16-2813. Epub 2017 Jul 27.
9
A p53/miR-30a/ZEB2 axis controls triple negative breast cancer aggressiveness.p53/miR-30a/ZEB2 轴控制三阴性乳腺癌的侵袭性。
Cell Death Differ. 2018 Dec;25(12):2165-2180. doi: 10.1038/s41418-018-0103-x. Epub 2018 Apr 17.
10
MiR-223-3p functions as a tumor suppressor in lung squamous cell carcinoma by miR-223-3p-mutant p53 regulatory feedback loop.miR-223-3p 通过 miR-223-3p 突变 p53 调节反馈环在肺鳞状细胞癌中作为肿瘤抑制因子发挥作用。
J Exp Clin Cancer Res. 2019 Feb 12;38(1):74. doi: 10.1186/s13046-019-1079-1.

引用本文的文献

1
MicroRNAs as Predictors of Future Uterine Malignancy in Endometrial Hyperplasia without Atypia.微小RNA作为非典型性子宫内膜增生未来发生子宫恶性肿瘤的预测指标
J Pers Med. 2022 Feb 18;12(2):311. doi: 10.3390/jpm12020311.
2
Rapid changes of miRNAs-20, -30, -410, -515, -134, and -183 and telomerase with psychological activity: A one year study on the relaxation response and epistemological considerations.miRNAs - 20、- 30、- 410、- 515、- 134和- 183以及端粒酶随心理活动的快速变化：一项关于放松反应和认识论考量的一年期研究。
J Tradit Complement Med. 2021 Feb 15;11(5):409-418. doi: 10.1016/j.jtcme.2021.02.005. eCollection 2021 Sep.
3

本文引用的文献

1
Extracellular matrix protein 1 regulates cell proliferation and trastuzumab resistance through activation of epidermal growth factor signaling.细胞外基质蛋白1通过激活表皮生长因子信号通路调节细胞增殖和曲妥珠单抗耐药性。
Breast Cancer Res. 2014 Dec 11;16(6):479. doi: 10.1186/s13058-014-0479-6.
2
Inhibition of triple-negative and Herceptin-resistant breast cancer cell proliferation and migration by Annexin A2 antibodies.膜联蛋白A2抗体对三阴性及曲妥珠单抗耐药乳腺癌细胞增殖和迁移的抑制作用
Br J Cancer. 2014 Dec 9;111(12):2328-41. doi: 10.1038/bjc.2014.542. Epub 2014 Oct 16.
3
MiRNA-30a inhibits AECs-II apoptosis by blocking mitochondrial fission dependent on Drp-1.
miR-138-5p induces aggressive traits by targeting Trp53 expression in murine melanoma cells, and correlates with poor prognosis of melanoma patients.
miR-138-5p 通过靶向 Trp53 表达诱导鼠黑色素瘤细胞的侵袭性表型，并与黑色素瘤患者的不良预后相关。
Neoplasia. 2021 Aug;23(8):823-834. doi: 10.1016/j.neo.2021.05.015. Epub 2021 Jul 8.
4
Cancer Testis Antigen, NOL4, Is an Immunogenic Antigen Specifically Expressed in Small-Cell Lung Cancer.癌睾丸抗原 NOL4 是一种在小细胞肺癌中特异性表达的免疫原性抗原。
Curr Oncol. 2021 May 20;28(3):1927-1937. doi: 10.3390/curroncol28030179.
5
MicroRNA-30a-3p functions as a tumor suppressor in renal cell carcinoma by targeting WNT2.微小RNA-30a-3p通过靶向WNT2在肾细胞癌中发挥肿瘤抑制作用。
Am J Transl Res. 2019 Aug 15;11(8):4976-4983. eCollection 2019.
6
iDEP: an integrated web application for differential expression and pathway analysis of RNA-Seq data.iDEP：一个用于 RNA-Seq 数据差异表达和通路分析的集成网络应用程序。
BMC Bioinformatics. 2018 Dec 19;19(1):534. doi: 10.1186/s12859-018-2486-6.
7
MiR-30a: A Novel Biomarker and Potential Therapeutic Target for Cancer.微小RNA-30a：一种新型癌症生物标志物及潜在治疗靶点
J Oncol. 2018 Aug 6;2018:5167829. doi: 10.1155/2018/5167829. eCollection 2018.
8
A p53/miR-30a/ZEB2 axis controls triple negative breast cancer aggressiveness.p53/miR-30a/ZEB2 轴控制三阴性乳腺癌的侵袭性。
Cell Death Differ. 2018 Dec;25(12):2165-2180. doi: 10.1038/s41418-018-0103-x. Epub 2018 Apr 17.
9
Identification of epigenetic interactions between miRNA and DNA methylation associated with gene expression as potential prognostic markers in bladder cancer.鉴定与基因表达相关的miRNA和DNA甲基化之间的表观遗传相互作用，作为膀胱癌潜在的预后标志物。
BMC Med Genomics. 2017 May 24;10(Suppl 1):30. doi: 10.1186/s12920-017-0269-y.
微小RNA-30a通过阻断依赖动力相关蛋白1（Drp-1）的线粒体分裂来抑制II型肺泡上皮细胞（AECs-II）凋亡。
J Cell Mol Med. 2014 Dec;18(12):2404-16. doi: 10.1111/jcmm.12420. Epub 2014 Oct 6.
4
MicroRNA-217 functions as a tumour suppressor gene and correlates with cell resistance to cisplatin in lung cancer.微小RNA-217作为一种肿瘤抑制基因发挥作用，并与肺癌细胞对顺铂的耐药性相关。
Mol Cells. 2014 Sep;37(9):664-71. doi: 10.14348/molcells.2014.0121. Epub 2014 Sep 18.
5
MiR-136 targets E2F1 to reverse cisplatin chemosensitivity in glioma cells.微小RNA-136靶向E2F1以逆转胶质瘤细胞对顺铂的化疗敏感性。
J Neurooncol. 2014 Oct;120(1):43-53. doi: 10.1007/s11060-014-1535-x. Epub 2014 Aug 20.
6
miR-326-histone deacetylase-3 feedback loop regulates the invasion and tumorigenic and angiogenic response to anti-cancer drugs.微小RNA-326-组蛋白去乙酰化酶-3反馈环调节对抗癌药物的侵袭、致瘤及血管生成反应。
J Biol Chem. 2014 Oct 3;289(40):28019-39. doi: 10.1074/jbc.M114.578229. Epub 2014 Aug 19.
7
MicroRNA expression profile of bromocriptine-resistant prolactinomas.溴隐亭抵抗性催乳素瘤的微小RNA表达谱
Mol Cell Endocrinol. 2014 Sep;395(1-2):10-8. doi: 10.1016/j.mce.2014.07.014. Epub 2014 Jul 23.
8
MicroRNA-30a promotes invasiveness and metastasis in vitro and in vivo through epithelial-mesenchymal transition and results in poor survival of nasopharyngeal carcinoma patients.微小RNA-30a通过上皮-间质转化在体外和体内促进侵袭和转移，并导致鼻咽癌患者生存率低下。
Exp Biol Med (Maywood). 2014 Jul;239(7):891-898. doi: 10.1177/1535370214532758. Epub 2014 May 8.
9
Exosomes from drug-resistant breast cancer cells transmit chemoresistance by a horizontal transfer of microRNAs.耐药乳腺癌细胞来源的外泌体通过微小RNA的水平转移传递化疗耐药性。
PLoS One. 2014 Apr 16;9(4):e95240. doi: 10.1371/journal.pone.0095240. eCollection 2014.
10
A microRNA signature associated with early recurrence in breast cancer.一种与乳腺癌早期复发相关的微小RNA特征。
PLoS One. 2014 Mar 14;9(3):e91884. doi: 10.1371/journal.pone.0091884. eCollection 2014.