Liu Lingqi, Chen Liang, Wu Tianpeng, Qian Huijun, Yang Sixing
Department of Urology, Renmin Hospital of Wuhan University Wuhan 430060, Hubei, China.
Am J Transl Res. 2019 Aug 15;11(8):4976-4983. eCollection 2019.
The expression and function of microRNA (miR)-30a-3p in several types of human cancer have been explored. However, the biological function of miR-30a-3p in renal cell carcinoma (RCC) remains largely unknown. In this study, we demonstrate that expression of miR-30a-3p is down-regulated in RCC tissues compared to adjacent normal tissues. Furthermore, ectopic expression of miR-30a-3p significantly suppressed the proliferation, migration, and invasion of a human RCC cell line , while miR-30a-3p inhibited tumor growth as well. TargetScan software identified Wnt2 as a potential direct target of miR-30a-3p. To confirm this relationship, Wnt2 was ectopically expressed. The effects of miR-30a-3p on RCC cell proliferation and invasion were subsequently restored. Therefore, the results of this study support an anti-tumor role for miR-30a-3p in RCC progression which is potentially mediated via Wnt2.
已经对微小RNA（miR）-30a-3p在几种人类癌症中的表达和功能进行了研究。然而，miR-30a-3p在肾细胞癌（RCC）中的生物学功能仍不清楚。在本研究中，我们证明与相邻正常组织相比，miR-30a-3p在RCC组织中的表达下调。此外，miR-30a-3p的异位表达显著抑制了人RCC细胞系的增殖、迁移和侵袭，同时miR-30a-3p也抑制了肿瘤生长。TargetScan软件将Wnt2鉴定为miR-30a-3p的潜在直接靶点。为了证实这种关系，Wnt2被异位表达。随后miR-30a-3p对RCC细胞增殖和侵袭的影响得以恢复。因此，本研究结果支持miR-30a-3p在RCC进展中具有抗肿瘤作用，这可能是通过Wnt2介导的。
