Tomson P L, Lumley P J, Smith A J, Cooper P R
Oral Biology, The University of Birmingham College of Medical and Dental Sciences, School of Dentistry, Birmingham, UK.
Int Endod J. 2017 Mar;50(3):281-292. doi: 10.1111/iej.12624. Epub 2016 Mar 14.
To characterize growth factor release from dentine by pulp-capping agents and to determine the effects of liberated dentine extracellular matrix (dECM) components on pulp cells in the key wound healing processes of migration and cell growth.
Powdered human dentine was exposed to solutions of calcium hydroxide, white and grey mineral trioxide aggregate (MTA) (ProRoot, (Dentsply Tulsa, Tulsa, OK, USA) over 14 days. The solubilized dECM components were dialysed and lyophilized and characterized using multiplex quantitative ELISA. Following dECM component extraction dentine was analysed using Fourier-transformed infrared spectroscopy (FTIR). Primary rat dental pulp cells (RDPCs) were exposed to dECM components (0.1-100 μg mL ) released by calcium hydroxide, white and grey MTA, and cell growth and chemotactic responses were assessed. Statistical differences between the experimental and control groups were determined using one-way anova.
A broad range of growth factors, many not previously reported in dentine, were liberated by these pulp-capping agents, including SCF, M-CSF, GM-CSF, IGFBP-1, NGF and GDNF. White and grey MTA liberated more growth factors than calcium hydroxide. FTIR analysis of dentine exposed to pulp-capping agents showed partial depletion of amide bands I, II and III, with little alteration in phosphate peaks compared to untreated dentine. dECM components released by white and grey MTA induced significantly more cell growth at low-to-moderate concentrations (P ≦ 0.05) examined in this study and significantly enhanced cell chemotaxis at all concentrations compared with controls (P ≦ 0.05).
White and grey MTA solubilize a broad range of bioactive molecules from dentine, which can induce proliferation and chemotaxis in pulp cells.
表征盖髓剂从牙本质中释放生长因子的情况，并确定释放的牙本质细胞外基质（dECM）成分在迁移和细胞生长等关键伤口愈合过程中对牙髓细胞的影响。
将人牙本质粉末暴露于氢氧化钙、白色和灰色三氧化矿物凝聚体（MTA）（ProRoot，美国俄克拉何马州塔尔萨市登士柏 Tulsa 公司）溶液中14天。将溶解的dECM成分进行透析和冻干，并使用多重定量酶联免疫吸附测定法进行表征。在提取dECM成分后，使用傅里叶变换红外光谱（FTIR）对牙本质进行分析。将原代大鼠牙髓细胞（RDPCs）暴露于由氢氧化钙、白色和灰色MTA释放的dECM成分（0.1 - 100μg/mL）中，并评估细胞生长和趋化反应。使用单因素方差分析确定实验组和对照组之间的统计学差异。
这些盖髓剂释放了多种生长因子，其中许多此前未在牙本质中报道过，包括干细胞因子（SCF）、巨噬细胞集落刺激因子（M - CSF）、粒细胞 - 巨噬细胞集落刺激因子（GM - CSF）、胰岛素样生长因子结合蛋白 - 1（IGFBP - 1）、神经生长因子（NGF）和胶质细胞源性神经营养因子（GDNF）。白色和灰色MTA释放的生长因子比氢氧化钙更多。对暴露于盖髓剂的牙本质进行FTIR分析显示，酰胺带I、II和III部分减少，与未处理的牙本质相比，磷酸盐峰变化不大。在本研究中检测的低至中等浓度下，白色和灰色MTA释放的dECM成分诱导的细胞生长显著更多（P≤0.05），并且与对照组相比，在所有浓度下均显著增强细胞趋化性（P≤0.05）。
白色和灰色MTA可从牙本质中溶解多种生物活性分子，这些分子可诱导牙髓细胞增殖和趋化。
