Oral Biology, The University of Birmingham College of Medical and Dental Sciences, School of Dentistry, St. Chad's Queensway, Birmingham B4 6NN, UK.
Cytokine. 2013 Feb;61(2):622-9. doi: 10.1016/j.cyto.2012.11.009. Epub 2012 Dec 28.
Extracellular matrix of dentine contains a rich cocktail of soluble cytokines and growth factors which mediate wound repair of the dentine-pulp complex. Hepatocyte growth factor (HGF) is a mesenchyme derived growth factor regulating a broad range of physiological processes including tissue development and regeneration. In this study, we have investigated the sequestration of HGF in the dentine matrix and analysed its action as a chemokine in the induction of differentiation and mineral deposition in pulp derived cells in vitro. Using ELISA, the presence of HGF was demonstrated in solubilised fractions of dentine matrix released by the therapeutic pulp repair materials of white and grey mineral trioxide aggregate. HGF was shown to be a chemo-attractant for primary rat dental pulp cells (RDPCs) in transwell assays highlighting its potential in progenitor cell recruitment during dentine-pulp tissue repair. Transcription factors Osterix and Runx2, and genes encoding for Osteopontin and Osteocalcin, were up-regulated in HGF-exposed RDPC cultures compared with controls. Adenoviral-mediated expression of HGF in RDPCs or exposure to recombinant HGF induced mineral secretion in RDPCs which was significantly greater than controls. The receptor of HGF, c-Met was also detected within human dental pulp indicating the potential for HGF released from dentine matrix to contribute to cellular signalling events following tissue injury. Combined, these data suggest that HGF is important in the repair of the dentine-pulp complex potentially participating in several aspects of wound healing.
牙本质的细胞外基质含有丰富的可溶性细胞因子和生长因子鸡尾酒，介导牙本质牙髓复合体的创伤修复。肝细胞生长因子（HGF）是一种间质衍生的生长因子，调节包括组织发育和再生在内的广泛生理过程。在这项研究中，我们研究了 HGF 在牙本质基质中的隔离及其作为趋化因子在体外诱导牙髓细胞分化和矿化沉积的作用。通过 ELISA，在白色和灰色矿物三氧化物聚合体治疗性牙髓修复材料释放的牙本质基质可溶部分中证明了 HGF 的存在。HGF 被证明是初级大鼠牙髓细胞（RDPC）在 Transwell 测定中的趋化因子，突出了其在牙髓组织修复过程中祖细胞募集中的潜在作用。与对照相比，HGF 暴露的 RDPC 培养物中转录因子 Osterix 和 Runx2 以及编码骨桥蛋白和骨钙素的基因上调。与对照相比，腺病毒介导的 RDPC 中 HGF 的表达或重组 HGF 的暴露诱导了 RDPC 中的矿化分泌，显著增加。HGF 的受体 c-Met 也在人牙髓中检测到，表明牙本质基质中释放的 HGF 可能参与组织损伤后的细胞信号事件。综上所述，这些数据表明 HGF 在牙本质牙髓复合体的修复中很重要，可能参与了创伤愈合的几个方面。
