Liang D W, Lu J H, Wu Q, Ke B X, Jiang C H, Long J, Fang Y P, Lin L J, Zeng N Y, Fu L, Jiang L X
Center of Clinical Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
Department of Laboratory Medicine, The First People's Hospital of Shunde District, Foshan, China.
J Appl Microbiol. 2016 Jun;120(6):1668-76. doi: 10.1111/jam.13106. Epub 2016 Apr 4.
To investigate whether the Luminex xMAP(®) Salmonella Serotyping Assay (xMAP SSA) is applicable to serotype Salmonella from humans in southern China.
Two hundred and five Salmonella isolates from diarrhoea patients were serotyped by xMAP SSA in parallel with the traditional serotyping. Forty serotypes were identified among 205 isolates; the most prevalent serotypes identified were Salmonella Enteritidis, Salmonella Stanley, Salmonella I 4,5,12:i:-, and Salmonella Typhimurium. One hundred and ninety-five (95·1%, 195/205) isolates were serotyped completely by xMAP SSA, while 10 stereotypes were partially detected as they were not included in the assay. The xMAP SSA correctly identified 192 (98·5%, 192/195) isolates. Five nonmotile and three monophasic strains, which possessed flagellar antigen genes that weren't expressed, were completely serotyped by xMAP SSA; however, these isolates were left undetected by the traditional method.
The xMAP SSA used in the study is a microsphere-based, molecular assay that could rapidly and accurately serotype Salmonella. It is suitable to identify the serovars of Salmonella in southern China.
The xMAP SSA, with high-throughput characteristics, provides an accurate and rapid serotyping system that dramatically strengthens the capability of clinical and public health laboratories for Salmonella serotyping.
研究Luminex xMAP(®)沙门氏菌血清分型检测法（xMAP SSA）是否适用于中国南方人类来源沙门氏菌的血清分型。
采用xMAP SSA对205株腹泻患者分离的沙门氏菌进行血清分型，并与传统血清分型方法同步进行。在205株分离菌中鉴定出40种血清型；最常见的血清型为肠炎沙门氏菌、斯坦利沙门氏菌、I 4,5,12:i:-沙门氏菌和鼠伤寒沙门氏菌。xMAP SSA完全鉴定出195株（95.1%，195/205）分离菌的血清型，而另外10种血清型因未包含在该检测方法中仅被部分检测到。xMAP SSA正确鉴定出192株（98.5%，192/195）分离菌。5株无动力菌和3株单相菌虽具有未表达的鞭毛抗原基因，但通过xMAP SSA完全鉴定出了血清型；然而，这些分离菌用传统方法未检测到。
本研究中使用的xMAP SSA是一种基于微球的分子检测方法，可快速、准确地对沙门氏菌进行血清分型。它适用于鉴定中国南方沙门氏菌的血清型。
具有高通量特性的xMAP SSA提供了一种准确、快速的血清分型系统，显著增强了临床和公共卫生实验室对沙门氏菌进行血清分型的能力。
