Lee K, Kinnunen M, Danilina A V, Ustinov V D, Shin S, Meglinski I, Priezzhev A V
Faculty of Physics, Lomonosov Moscow State University, Moscow, Russia; Opto-Electronics and Measurement Techniques Research Unit, University of Oulu, Oulu, Finland.
Opto-Electronics and Measurement Techniques Research Unit, University of Oulu, Oulu, Finland.
J Biomech. 2016 May 3;49(7):1021-1026. doi: 10.1016/j.jbiomech.2016.02.011. Epub 2016 Feb 13.
The aggregation of red blood cells (RBC) is an intrinsic feature of blood that has a strong impact on its microcirculation. For a number of years it has been attracting a great attention in basic research and clinical studies. Here, we study a relationship between the RBC aggregation parameters measured at the individual cell level and in a whole blood sample. The home made optical tweezers were used to measure the aggregating and disaggregating forces for a pair of interacting RBCs, at the individual cell level, in order to evaluate the corresponding shear stresses. The RheoScan aggregometer was used for the measurements of critical shear stress (CSS) in whole blood samples. The correlation between CSS and the shear stress required to stop an RBC pair from aggregating was found. The shear stress required to disaggregate a pair of RBCs using the double channel optical tweezers appeared to be about 10 times higher than CSS. The correlation between shear stresses required to prevent RBCs from aggregation at the individual cell level and in whole blood samples was estimated and assessed quantitatively. The experimental approach developed has a high potential for advancing hemorheological studies.
红细胞（RBC）的聚集是血液的一种内在特性，对其微循环有很大影响。多年来，它在基础研究和临床研究中一直备受关注。在此，我们研究在单个细胞水平和全血样本中测量的红细胞聚集参数之间的关系。使用自制的光镊在单个细胞水平测量一对相互作用的红细胞的聚集和解聚力，以评估相应的剪切应力。流变扫描聚集仪用于测量全血样本中的临界剪切应力（CSS）。发现了CSS与阻止一对红细胞聚集所需的剪切应力之间的相关性。使用双通道光镊使一对红细胞解聚所需的剪切应力似乎比CSS高约10倍。定量估计并评估了在单个细胞水平和全血样本中防止红细胞聚集所需的剪切应力之间的相关性。所开发的实验方法在推进血液流变学研究方面具有很大潜力。
