Diaz-Gomez Luis, Concheiro Angel, Alvarez-Lorenzo Carmen, García-González Carlos A
Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia, Universidade de Santiago de Compostela, E-15782 Santiago de Compostela, Spain.
Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia, Universidade de Santiago de Compostela, E-15782 Santiago de Compostela, Spain.
Carbohydr Polym. 2016 May 20;142:282-92. doi: 10.1016/j.carbpol.2016.01.051. Epub 2016 Jan 25.
Synthetic polymeric scaffolds to be used as surrogates of autologous bone grafts should not only have suitable physicochemical and mechanical properties, but also contain bioactive agents such as growth factors (GFs) to facilitate the tissue growth. For this purpose, cost-effective and autologous GFs sources are preferred to avoid some post-surgery complications after implantation, like immunogenicity or disease transmission, and the scaffolds should be processed using methods able to preserve GFs activity. In this work, poly(ɛ-caprolactone) (PCL) scaffolds incorporating GFs were processed using a green foaming process based on supercritical fluid technology. Preparation rich in growth factors (PRGF), a natural and highly available cocktail of GFs obtained from platelet rich plasma (PRP), was used as GF source. PCL:starch:PRGF (85:10:5 weight ratio) porous solid scaffolds were obtained by a supercritical CO2-assisted foaming process at 100 bar and 37 °C with no need of post-processing steps. Bioactivity of GFs after processing and scaffold cytocompatibility were confirmed using mesenchymal stem cells. The performance of starch as GF control release component was shown to be dependent on starch pre-gelification conditions.
用作自体骨移植替代物的合成聚合物支架不仅应具有合适的物理化学和机械性能，还应含有生物活性剂，如生长因子（GFs），以促进组织生长。为此，首选具有成本效益的自体生长因子来源，以避免植入后出现一些术后并发症，如免疫原性或疾病传播，并且支架应采用能够保持生长因子活性的方法进行加工。在这项工作中，基于超临界流体技术的绿色发泡工艺对含有生长因子的聚（ε-己内酯）（PCL）支架进行了加工。富含生长因子的血浆（PRGF）是一种从富含血小板的血浆（PRP）中获得的天然且易于获取的生长因子混合物，用作生长因子来源。通过在100巴和37°C下的超临界CO2辅助发泡工艺，无需后处理步骤，即可获得PCL:淀粉:PRGF（重量比为85:10:5）的多孔固体支架。使用间充质干细胞确认了加工后生长因子的生物活性和支架的细胞相容性。结果表明，淀粉作为生长因子控释成分的性能取决于淀粉的预凝胶化条件。
