Benslimane Yahya, Harrington Lea
Department of Molecular Biology, University of Montreal, Institute for Research in Immunology and Cancer, Montreal, Quebec, Canada.
Department of Molecular Biology, University of Montreal, Institute for Research in Immunology and Cancer, Montreal, Quebec, Canada; Department of Biochemistry, University of Montreal, Institute for Research in Immunology and Cancer, Montreal, Quebec, Canada; Department of Medicine, University of Montreal, Institute for Research in Immunology and Cancer, Montreal, Quebec, Canada.
F1000Res. 2015 Dec 11;4. doi: 10.12688/f1000research.6664.1. eCollection 2015.
Fluorescence microscopy can be used to assess the dynamic localization and intensity of single entities in vitro or in living cells. It has been applied with aplomb to many different cellular processes and has significantly enlightened our understanding of the heterogeneity and complexity of biological systems. Recently, high-resolution fluorescence microscopy has been brought to bear on telomeres, leading to new insights into telomere spatial organization and accessibility, and into the mechanistic nuances of telomere elongation. We provide a snapshot of some of these recent advances with a focus on mammalian systems, and show how three-dimensional, time-lapse microscopy and single-molecule fluorescence shine a new light on the end of the chromosome.
荧光显微镜可用于评估体外或活细胞中单个实体的动态定位和强度。它已被成功应用于许多不同的细胞过程，并极大地增进了我们对生物系统异质性和复杂性的理解。最近，高分辨率荧光显微镜已应用于端粒研究，从而对端粒的空间组织、可及性以及端粒延长的机制细微差别有了新的认识。我们重点介绍哺乳动物系统中这些最新进展的一些情况，并展示三维延时显微镜和单分子荧光如何为染色体末端带来新的认识。
