Li Helin, Gao Rui, Zhang Yanming
1 College of Veterinary Medicine, Northwest A&F University , Yangling, Shaanxi, P.R. China .
2 Yangling Vocational & Technical College , Yangling, Shaanxi, P.R. China .
Viral Immunol. 2016 May;29(4):244-51. doi: 10.1089/vim.2015.0068. Epub 2016 Feb 26.
Classical swine fever (CSF) vaccine based on HAdV-5 had achieved an efficient protection in swine. Both classical swine fever virus (CSFV) E0 glycoprotein and E2 glycoprotein were the targets for neutralizing antibodies and related to immune protection against CSF. Interleukin-2 (IL2), as an adjuvant, also had been used in CSF vaccine research. In this study, coexpression of the CSFV E0, E2, and IL2 genes by HAdV-5 (rAdV-E0-E2-IL2) was constructed and immunized to evaluate its efficacy. Three expressed genes had been sequentially connected with foot-and-mouth disease virus 2A (FMDV 2A). The vaccine was administered by intramuscular inoculation to CSFV-free pigs (10(8) TCID50) twice at triweekly intervals. No adverse clinical signs were observed in any of the pigs after vaccination. The vaccine induced strong humoral and cellular responses that led to complete protection against clinical signs of lethal CSFV infection, viremia, and shedding of challenge virus. The rAdV-E0-E2-IL2 is a promising, efficient, and safe marker vaccine candidate against CSFV.
基于人腺病毒5型（HAdV-5）的经典猪瘟（CSF）疫苗已在猪中实现了高效保护。经典猪瘟病毒（CSFV）E0糖蛋白和E2糖蛋白都是中和抗体的靶标，且与针对CSF的免疫保护相关。白细胞介素-2（IL2）作为佐剂，也已用于CSF疫苗研究。在本研究中，构建了由HAdV-5共表达CSFV E0、E2和IL2基因（rAdV-E0-E2-IL2）并进行免疫以评估其效力。三个表达的基因已通过口蹄疫病毒2A（FMDV 2A）依次连接。该疫苗通过肌肉注射接种给无CSFV的猪（10^8 TCID50），每隔一周注射两次。接种疫苗后，在任何猪中均未观察到不良临床症状。该疫苗诱导了强烈的体液和细胞反应，从而对致死性CSFV感染的临床症状、病毒血症和攻毒病毒的排出提供了完全保护。rAdV-E0-E2-IL2是一种有前景、高效且安全的抗CSFV标记疫苗候选物。
