Xia Shui-Li, Xiang Guang-Tao, Lei Jian-Lin, Du Mingliang, Wang Yimin, Zhou Mo, Liu Yan, Ji Shengwei, Wang Ya-Lin, Luo Yuzi, Sun Yuan, Qiu Hua-Ji
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China.
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China.
Vet Microbiol. 2016 Nov 30;196:50-54. doi: 10.1016/j.vetmic.2016.10.001. Epub 2016 Oct 5.
Classical swine fever (CSF) is an economically important disease caused by Classical swine fever virus (CSFV). In order to eradicate CSF, many marker vaccines that allow differentiation of infected from vaccinated animals (DIVA) have been developed. In our previous studies, a DIVA CSF vaccine rAdV-SFV-E2 has been demonstrated to completely protect pigs against lethal CSFV challenge. In the context of risk assessments for an emergency vaccination scenario, the question has been raised whether preexisting maternally derived antibodies (MDAs) interfere with the efficacy of the vaccine. In this study, six groups of piglets (n=5), with or without anti-C-strain or anti-rAdV-SFV-E2 MDAs, were immunized twice with 10 TCID rAdV-SFV-E2 and challenged with the CSFV Shimen strain. Clinical signs, CSFV-specific antibodies, viremia and pathological and histopathological changes were monitored. The results showed that the vaccinated piglets, either with or without MDAs directed against C-strain (about 67% blocking rate) or rAdV-SFV-E2 (about 50% blocking rate) were completely protected; however, the mock-vaccinated piglets displayed severe CSF-typical clinical symptoms, viremia, pathological/histopathological changes and deaths (5/5). These findings demonstrate that the MDAs to either rAdV-SFV-E2 or C-strain do not interfere with the efficacy of rAdV-SFV-E2, which highlights the great potential of the vaccine for control and eradication of CSF.
经典猪瘟（CSF）是由经典猪瘟病毒（CSFV）引起的一种具有重要经济影响的疾病。为根除经典猪瘟，已研发出许多能够区分感染动物和免疫动物（DIVA）的标记疫苗。在我们之前的研究中，一种DIVA猪瘟疫苗rAdV - SFV - E2已被证明能完全保护猪免受致死性CSFV攻击。在紧急免疫接种情况的风险评估背景下，有人提出母体来源抗体（MDA）是否会干扰疫苗效力的问题。在本研究中，六组仔猪（每组n = 5），有或没有抗C株或抗rAdV - SFV - E2的MDA，用10个TCID的rAdV - SFV - E2免疫两次，并用CSFV石门株进行攻毒。监测临床症状、CSFV特异性抗体、病毒血症以及病理和组织病理学变化。结果显示，接种疫苗的仔猪，无论有无针对C株（阻断率约67%）或rAdV - SFV - E2（阻断率约50%）的MDA，均得到完全保护；然而，未接种疫苗的仔猪出现严重的典型猪瘟临床症状、病毒血症、病理/组织病理学变化并死亡（5/5）。这些发现表明，针对rAdV - SFV - E2或C株的MDA不会干扰rAdV - SFV - E2的效力，这突出了该疫苗在控制和根除猪瘟方面的巨大潜力。
