Dahan Julien M, Giron Celine, Concordet Didier, Dossin Olivier
Am J Vet Res. 2016 Mar;77(3):288-93. doi: 10.2460/ajvr.77.3.288.
To evaluate circadian and postprandial variations in plasma citrulline concentration in healthy dogs.
8 healthy Beagles.
Blood samples were collected from dogs after 12 hours of food withholding (0 hours; 8:00 am) and then every 2 hours for 12 hours (until 8:00 pm) and again at 24 hours (8:00 am the next day). The same protocol was repeated, with the only difference being that a meal was given immediately after the 0-hour sample collection point. Plasma citrulline concentration was measured by ion exchange chromatography.
No significant difference in plasma citrulline concentration was identified among measurement points when food was withheld. Mean ± SD plasma citrulline concentration at 4 hours (72.2 ± 12.7 μmol/L) and 24 hours (56.1 ± 12.5 μmol/L) after dogs were fed was significantly different from that at 0 hours (64.4 ± 12.7 μmol/L).
Plasma citrulline concentration had no circadian variation in unfed dogs but increased significantly in fed dogs 4 hours after a meal. Therefore, food should be withheld from dogs for 8 to 12 hours before blood sample collection for measurement of citrulline concentration.
评估健康犬血浆瓜氨酸浓度的昼夜节律和餐后变化。
8只健康比格犬。
在禁食12小时后（0小时；上午8:00）从犬只采集血样，然后每2小时采集一次，共采集12小时（直至晚上8:00），并在24小时（第二天上午8:00）再次采集。重复相同方案，唯一不同的是在0小时采样点采集后立即喂食。采用离子交换色谱法测定血浆瓜氨酸浓度。
禁食时各测量点血浆瓜氨酸浓度无显著差异。犬只进食后4小时（72.2±12.7μmol/L）和24小时（56.1±12.5μmol/L）的血浆瓜氨酸浓度平均值±标准差与0小时（64.4±12.7μmol/L）有显著差异。
未进食犬的血浆瓜氨酸浓度无昼夜节律变化，但进食犬在进食后4小时显著升高。因此，在采集血样测定瓜氨酸浓度前，应让犬禁食8至12小时。
