Ning Dandan, Duong Binh, Thomas Gawain, Qiao Yong, Ma Liyuan, Wen Qi, Su Ming
Wenzhou Institute of Biomaterials and Engineering, Chinese Academy of Sciences , Wenzhou Medical University, Wenzhou, Zhejiang 325001, People's Republic of China.
Department of Chemical Engineering, Northeastern University , Boston, Massachusetts 02115, United States.
Langmuir. 2016 Mar 22;32(11):2718-23. doi: 10.1021/acs.langmuir.5b04469. Epub 2016 Mar 7.
Cancer metastasis is a major cause of cancer-induced deaths in patients. Mimicking nanostructures of an extracellular matrix surrounding cancer cells can provide useful clues for metastasis. This paper compares the morphology, proliferation, spreading, and stiffness of highly aggressive glioblastoma multiforme cancer cells and normal fibroblast cells seeded on a variety of ordered polymeric nanostructures (nanopillars and nanochannels). Both cell lines survive and proliferate on the nanostructured surface and show more similarity on nanostructured surfaces than on flat surfaces. Although both show similar stiffness on the nanochannel surface, glioblastomas are softer, spread to a larger area, and elongate less than fibroblasts. The nanostructured surfaces are useful for in vitro model of an extracellular matrix to study the cancer cell migratory phenotype.
癌症转移是导致癌症患者死亡的主要原因。模拟癌细胞周围细胞外基质的纳米结构可为转移研究提供有用线索。本文比较了接种在各种有序聚合物纳米结构（纳米柱和纳米通道）上的高侵袭性多形性胶质母细胞瘤癌细胞和正常成纤维细胞的形态、增殖、铺展及硬度。两种细胞系均可在纳米结构表面存活和增殖，且在纳米结构表面比在平坦表面表现出更多相似性。虽然在纳米通道表面二者表现出相似的硬度，但胶质母细胞瘤细胞更柔软，铺展面积更大，且伸长程度小于成纤维细胞。纳米结构表面对于研究癌细胞迁移表型的细胞外基质体外模型很有用。
