Harberts Jann, Zierold Robert, Fendler Cornelius, Koitmäe Aune, Bayat Parisa, Fernandez-Cuesta Irene, Loers Gabriele, Diercks Björn-Philipp, Fliegert Ralf, Guse Andreas H, Ronning Carsten, Otnes Gaute, Borgström Magnus, Blick Robert H
Center for Hybrid Nanostructures, Universität Hamburg Luruper Chaussee 149 22761 Hamburg Germany
Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf Falkenried 94 20251 Hamburg Germany.
RSC Adv. 2019 Apr 9;9(20):11194-11201. doi: 10.1039/c8ra05320k.
Nanowire substrates play an increasingly important role for cell cultures as an approach for hybrid bio-semiconductor junctions. We investigate Jurkat T cells and neurons from mice cultured on AlO coated ordered and randomly distributed nanowires. Cell viability was examined by life/membrane staining reporting comparable viability on planar and nanowire substrates. Imaging the hybrid interface reveals a wrapping of the cell membrane around the very nanowire tip. Patch clamp recordings show similar electrophysiological responses on each type of nanowires compared to planar control substrates. We demonstrate that the morphological characteristic of the nanowire substrate plays a subordinate role which opens up the arena for a large range of nanowire substrates in a functionalized application such as stimulation or sensing.
纳米线基底作为一种用于混合生物 - 半导体结的方法，在细胞培养中发挥着越来越重要的作用。我们研究了在涂覆有AlO的有序和随机分布的纳米线上培养的小鼠Jurkat T细胞和神经元。通过活/膜染色检查细胞活力，结果表明在平面和纳米线基底上的细胞活力相当。对混合界面进行成像显示细胞膜围绕纳米线尖端缠绕。膜片钳记录表明，与平面对照基底相比，每种类型的纳米线上的电生理反应相似。我们证明纳米线基底的形态特征起次要作用，这为功能化应用（如刺激或传感）中的各种纳米线基底开辟了广阔的应用前景。
