从甜菜糖蜜中分离出的新型寡糖的结构确证
Structural confirmation of novel oligosaccharides isolated from sugar beet molasses.
作者信息
Abe Tatsuya, Kikuchi Hiroto, Aritsuka Tsutomu, Takata Yusuke, Fukushi Eri, Fukushi Yukiharu, Kawabata Jun, Ueno Keiji, Onodera Shuichi, Shiomi Norio
机构信息
Research Center, Nippon Beet Sugar Mfg. Co., Ltd., Obihiro 080-0831, Japan.
Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
出版信息
Food Chem. 2016 Jul 1;202:284-90. doi: 10.1016/j.foodchem.2016.01.126. Epub 2016 Jan 28.
Eleven oligosaccharides were isolated from sugar beet molasses using carbon-Celite column chromatography and HPLC. The constituent sugars and linkage positions were determined using methylation analysis, MALDI-TOF-MS, and NMR measurements. The configurations of isolated oligosaccharides were confirmed based on detailed NMR analysis. Based on our results, three of the 11 oligosaccharides were novel.
使用碳 - 硅藻土柱色谱法和高效液相色谱法从甜菜糖蜜中分离出11种低聚糖。通过甲基化分析、基质辅助激光解吸电离飞行时间质谱（MALDI - TOF - MS）和核磁共振测量来确定组成糖和连接位置。基于详细的核磁共振分析确认了分离出的低聚糖的构型。根据我们的结果，11种低聚糖中有3种是新的。
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