Okamoto Eiji, Kikuchi Sakiko, Mitamura Yoshinori
Graduate School of Science and Engineering, Tokai University, Minami-sawa 5-1-1-1, Minami-ku, Sapporo, 005-8601, Japan.
J Artif Organs. 2016 Sep;19(3):257-61. doi: 10.1007/s10047-016-0889-6. Epub 2016 Feb 26.
We have developed a tissue-inducing electrode using titanium mesh to obtain mechanically and electrically stable contact with the tissue for a new transcutaneous communication system using the human body as a conductive medium. In this study, we investigated the electrical properties of the titanium mesh electrode by measuring electrode-tissue interface resistance in vivo. The titanium mesh electrode (Hi-Lex Co., Zellez, Hyogo, Japan) consisted of titanium fibers (diameter of 50 μm), and it has an average pore size of 200 μm and 87 % porosity. The titanium mesh electrode has a diameter of 5 mm and thickness of 1.5 mm. Three titanium mesh electrodes were implanted separately into the dorsal region of the rat. We measured the electrode-electrode impedance using an LCR meter for 12 weeks, and we calculated the tissue resistivity and electrode-tissue interface resistance. The electrode-tissue interface resistance of the titanium mesh electrode decreased slightly until the third POD and then continuously increased to 75 Ω. The electrode-tissue interface resistance of the titanium mesh electrode is stable and it has lower electrode-tissue interface resistance than that of a titanium disk electrode. The extracted titanium mesh electrode after 12 weeks implantation was fixed in 10 % buffered formalin solution and stained with hematoxylin-eosin. Light microscopic observation showed that the titanium mesh electrode was filled with connective tissue, inflammatory cells and fibroblasts with some capillaries in the pores of the titanium mesh. The results indicate that the titanium mesh electrode is a promising electrode for the new transcutaneous communication system.
我们开发了一种使用钛网的组织诱导电极，以便在一种以人体作为导电介质的新型经皮通信系统中与组织实现机械和电气上的稳定接触。在本研究中，我们通过在体内测量电极 - 组织界面电阻来研究钛网电极的电学性质。钛网电极（日本兵库县佐用市Hi-Lex公司）由直径为50μm的钛纤维组成，平均孔径为200μm，孔隙率为87%。钛网电极直径为5mm，厚度为1.5mm。将三个钛网电极分别植入大鼠背部区域。我们使用LCR测量仪测量电极 - 电极阻抗，持续12周，并计算组织电阻率和电极 - 组织界面电阻。钛网电极的电极 - 组织界面电阻在术后第3天之前略有下降，然后持续增加至75Ω。钛网电极的电极 - 组织界面电阻稳定，并且其电极 - 组织界面电阻低于钛盘电极。植入12周后取出的钛网电极固定在10%的缓冲福尔马林溶液中，并用苏木精 - 伊红染色。光学显微镜观察显示，钛网电极孔隙内充满结缔组织、炎性细胞和成纤维细胞，并有一些毛细血管。结果表明，钛网电极对于新型经皮通信系统是一种很有前景的电极。
