Ichinohe Norihisa, Kuboki Yoshinori, Tabata Yasuhiko
Department of Biomaterials, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan.
Tissue Eng Part A. 2008 Oct;14(10):1663-71. doi: 10.1089/ten.tea.2006.0350.
The objective of this study was to modify titanium nonwoven fabrics (Ti) with a hydroxyapatite (HA)-like coating and fibroblast growth factor (FGF)-2 combination, and evaluate the bone regeneration potential of the modified Ti. Biodegradable gelatin hydrogel microspheres (GM) were prepared as a carrier matrix for the controlled release of FGF-2. Ti, HA-coated Ti (Ti-HA), and Ti-HA incorporating GM (Ti-HA-GM) infused FGF-2 were applied to skull defects of rabbits. Then osteointegration in the Ti was evaluated by alkaline phosphatase activity, Ca(2+) content, and histological observation, and the hemoglobin content was assessed for angiogenic measurement. Ti-HA-GM promoted bone regeneration to a significantly greater extent than Ti, Ti-HA, or mixed Ti-HA and free FGF-2 6 weeks after application, and it also enhanced the hemoglobin content. It is concluded that the combination of HA-like coating and FGF-2 release promotes Ti induction of bone regeneration.
本研究的目的是用类羟基磷灰石（HA）涂层和成纤维细胞生长因子（FGF）-2组合对钛无纺布（Ti）进行改性，并评估改性Ti的骨再生潜力。制备了可生物降解的明胶水凝胶微球（GM）作为FGF-2控释的载体基质。将Ti、HA涂层Ti（Ti-HA）和掺入GM的Ti-HA（Ti-HA-GM）注入FGF-2应用于兔颅骨缺损。然后通过碱性磷酸酶活性、Ca(2+)含量和组织学观察评估Ti中的骨整合，并评估血红蛋白含量以进行血管生成测量。应用6周后，Ti-HA-GM促进骨再生的程度明显大于Ti、Ti-HA或混合的Ti-HA和游离FGF-2，并且还提高了血红蛋白含量。得出结论，类HA涂层和FGF-2释放的组合促进了Ti诱导的骨再生。
