Pizarro Consuelo, Arenzana-Rámila Irene, Pérez-del-Notario Nuria, Pérez-Matute Patricia, González-Sáiz José María
Department of Chemistry, University of La Rioja, C/ Madre de Dios 51, 26006, Logroño, La Rioja, Spain.
Department of Chemistry, University of La Rioja, C/ Madre de Dios 51, 26006, Logroño, La Rioja, Spain.
Anal Chim Acta. 2016 Mar 17;912:1-9. doi: 10.1016/j.aca.2016.01.058. Epub 2016 Feb 4.
Lipid profiling is a promising tool for the discovery and subsequent identification of biomarkers associated with various diseases. However, data quality is quite dependent on the pre-analytical methods employed. To date, potential confounding factors that may affect lipid metabolite levels after the thawing of plasma for biomarker exploration studies have not been thoroughly evaluated. In this study, by means of experimental design methodology, we performed the first in-depth examination of the ways in which thawing conditions affect lipid metabolite levels. After the optimization stage, we concluded that temperature, sample volume and the thawing method were the determining factors that had to be exhaustively controlled in the thawing process to ensure the quality of biomarker discovery. Best thawing conditions were found to be: 4 °C, with 0.25 mL of human plasma and ultrasound (US) thawing. The new US proposed thawing method was quicker than the other methods we studied, allowed more features to be identified and increased the signal of the lipids. In view of its speed, efficiency and detectability, the US thawing method appears to be a simple, economical method for the thawing of plasma samples, which could easily be applied in clinical laboratories before lipid profiling studies.
脂质谱分析是发现并随后鉴定与各种疾病相关生物标志物的一种很有前景的工具。然而，数据质量在很大程度上取决于所采用的分析前方法。迄今为止，对于生物标志物探索研究中血浆解冻后可能影响脂质代谢物水平的潜在混杂因素尚未进行全面评估。在本研究中，我们借助实验设计方法，首次深入研究了解冻条件影响脂质代谢物水平的方式。经过优化阶段后，我们得出结论，温度、样本体积和解冻方法是解冻过程中必须严格控制的决定因素，以确保生物标志物发现的质量。发现最佳解冻条件为：4℃，使用0.25 mL人血浆并采用超声（US）解冻。新提出的超声解冻方法比我们研究的其他方法更快，能够鉴定出更多特征并增强脂质信号。鉴于其速度、效率和可检测性，超声解冻方法似乎是一种简单、经济的血浆样本解冻方法，可轻松应用于脂质谱分析研究前的临床实验室。
