Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang, China.
School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia.
J Infect. 2016 May;72(5):564-72. doi: 10.1016/j.jinf.2016.02.005. Epub 2016 Feb 23.
Cholera is potentially a life threatening disease caused by toxigenic Vibrio cholerae. Here we report the identification and characterisation of 76 non-7th pandemic clone O1 V. cholerae isolates including 65 clinical isolates from diarrhoeal patients from 2005 to 2014 in Zhejiang Province, China.
We used multilocus sequence typing (MLST) to characterise 65 V. cholerae isolates. Pulse-Field Gel Electrophoresis (PFGE) was performed on a subset of the isolates and whole-genome sequencing was done on 13 isolates.
MLST separated 65 isolates into 19 sequence types (STs). Thirty three isolates belonged to ST75 which also contains the US Gulf Coast clone. PFGE separated the 33 isolates into 16 pulsotypes. Whole genome sequencing of 10 ST75 isolates showed that the US Gulf Coast clone and the Chinese ST75 isolates can be separated into two distinct lineages, ST75a and ST75b. All Zhejiang ST75 isolates were ST75b.
PFGE and genome sequencing confirmed the linked cases and identified small outbreaks caused by ST75b. The emergence and potential spread of ST75b may pose significant threat to public health. Epidemiological surveillance is required to further understand its epidemic potential.
霍乱是一种由产毒霍乱弧菌引起的潜在致命疾病。本研究报告了 76 株非 7 型大流行克隆 O1 霍乱弧菌的鉴定和特征，包括 2005 年至 2014 年期间来自中国浙江省腹泻患者的 65 株临床分离株。
我们使用多位点序列分型（MLST）对 65 株霍乱弧菌进行了特征分析。选择部分分离株进行脉冲场凝胶电泳（PFGE）分析，并对 13 株分离株进行全基因组测序。
MLST 将 65 株分离株分为 19 个序列型（ST）。33 株分离株属于 ST75，其中还包含美国墨西哥湾沿岸克隆株。PFGE 将 33 株分离株分为 16 个脉冲型。对 10 株 ST75 分离株的全基因组测序表明，美国墨西哥湾沿岸克隆株和中国 ST75 分离株可分为两个不同的谱系，ST75a 和 ST75b。所有浙江 ST75 分离株均为 ST75b。
PFGE 和基因组测序证实了相关病例，并鉴定出由 ST75b 引起的小型暴发。ST75b 的出现和潜在传播可能对公共卫生构成重大威胁。需要进行流行病学监测以进一步了解其流行潜力。
