National Infection Services, Public Health England, London, United Kingdom.
National Public Health Laboratory, Ministry of Health, Singapore, Singapore.
J Clin Microbiol. 2018 Oct 25;56(11). doi: 10.1128/JCM.00831-18. Print 2018 Nov.
Epidemiological and microbiological data on strains isolated between April 2004 and March 2018 ( = 836) and held at the Public Health England culture archive were reviewed. The traditional biochemical species identification and serological typing results were compared with the genome-derived species identification and serotype for a subset of isolates ( = 152). Of the 836 isolates, 750 (89.7%) were from a fecal specimen, 206 (24.6%) belonged to serogroup O1, and 7 (0.8%) were serogroup O139; 792 (94.7%) isolates were from patients reporting recent travel abroad, most commonly to India ( = 209) and Pakistan ( = 104). Of the 152 isolates identified by use of kmer, 149 (98.1%) were concordant with those identified using the traditional biochemical approach. Traditional serotyping results were 100% concordant with those of the whole-genome sequencing (WGS) analysis for the identification of serogroups O1 and O139 and classical and El Tor biotypes. was detected in all isolates of O1 El Tor and O139 belonging to sequence type 69 (ST69) and in O1 classical variants belonging to ST73. A phylogeny of isolates belonging to ST69 from U.K. travelers clustered geographically, with isolates from India and Pakistan located on separate branches. Moving forward, WGS data from U.K. travelers will contribute to global surveillance programs and the monitoring of emerging threats to public health and the global dissemination of pathogenic lineages. At the national level, these WGS data will inform the timely reinforcement of direct public health messaging to travelers and mitigate the impact of imported infections and the associated risks to public health.
对 2004 年 4 月至 2018 年 3 月（= 836）期间分离并保存在英国公共卫生署培养物档案中的菌株的流行病学和微生物学数据进行了回顾。比较了传统生化种鉴定和血清型分型结果与基因组衍生种鉴定和血清型对一组分离株（= 152）的结果。在 836 株分离株中，750 株（89.7%）来自粪便标本，206 株（24.6%）属于 O1 血清群，7 株（0.8%）属于 O139 血清群；792 株（94.7%）分离株来自报告近期出国旅行的患者，最常见的是印度（= 209）和巴基斯坦（= 104）。使用 kmer 鉴定的 152 株分离株中，149 株（98.1%）与传统生化方法鉴定的结果一致。传统血清分型结果与全基因组测序（WGS）分析对 O1 和 O139 血清群以及经典和 El Tor 生物型的鉴定完全一致。所有属于序列型 69（ST69）的 O1 El Tor 和 O139 分离株以及属于 ST73 的 O1 经典变体均检测到 。来自英国旅行者的属于 ST69 的分离株的系统发育聚类在地理上，来自印度和巴基斯坦的分离株位于不同的分支上。未来，来自英国旅行者的 WGS 数据将有助于全球监测计划和对公共卫生新兴威胁的监测以及致病谱系的全球传播。在国家层面，这些 WGS 数据将及时为旅行者提供公共卫生信息，减轻输入性感染的影响及其对公共卫生的相关风险。
