Bochi Guilherme Vargas, Torbitz Vanessa Dorneles, de Campos Luízi Prestes, Sangoi Manuela Borges, Fernandes Natieli Flores, Gomes Patrícia, Moretto Maria Beatriz, Barbisan Fernanda, da Cruz Ivana Beatrice Mânica, Moresco Rafael Noal
Laboratory of Clinical Biochemistry, Department of Clinical and Toxicological Analysis, Health Sciences Center, Federal University of Santa Maria, Santa Maria, Brazil.
Pharmacology Postgraduate Program, Health Sciences Center, Federal University of Santa Maria, Santa Maria, Brazil.
Inflammation. 2016 Apr;39(2):916-27. doi: 10.1007/s10753-016-0325-3.
The accumulation of advanced oxidation protein products (AOPPs) has been linked to several pathological conditions. Here, we investigated collagen as a potential source for AOPP formation and determined the effects of hypochlorous acid (HOCl)-treated collagen (collagen-AOPPs) on human neutrophil activity. We also assessed whether alpha-tocopherol could counteract these effects. Exposure to HOCl increased the levels of collagen-AOPPs. Collagen-AOPPs also stimulated the production of AOPPs, nitric oxide (NO), superoxide radicals (O2(-)), and HOCl by neutrophils. Collagen-AOPPs induced apoptosis and decreased the number of viable cells. Alpha-tocopherol prevented the formation of collagen-AOPPs, strongly inhibited the collagen-AOPP-induced production of O2(-) and HOCl, and increased the viability of neutrophils. Our results suggest that collagen is an important protein that interacts with HOCl to form AOPPs, and consequently, collagen-AOPP formation is related to human neutrophil activation and cell death.
晚期氧化蛋白产物（AOPPs）的积累与多种病理状况有关。在此，我们研究了胶原蛋白作为AOPP形成的潜在来源，并确定了次氯酸（HOCl）处理的胶原蛋白（胶原蛋白-AOPPs）对人中性粒细胞活性的影响。我们还评估了α-生育酚是否能抵消这些影响。暴露于HOCl会增加胶原蛋白-AOPPs的水平。胶原蛋白-AOPPs还刺激中性粒细胞产生AOPPs、一氧化氮（NO）、超氧自由基（O2(-)）和HOCl。胶原蛋白-AOPPs诱导细胞凋亡并减少活细胞数量。α-生育酚可防止胶原蛋白-AOPPs的形成，强烈抑制胶原蛋白-AOPPs诱导的O2(-)和HOCl的产生，并增加中性粒细胞的活力。我们的结果表明，胶原蛋白是一种重要的蛋白质，它与HOCl相互作用形成AOPPs，因此，胶原蛋白-AOPPs的形成与人中性粒细胞的激活和细胞死亡有关。
