Pablos-Tanarro Alba, López-Expósito Ivan, Lozano-Ojalvo Daniel, López-Fandiño Rosina, Molina Elena
Instituto de Investigación en Ciencias de la Alimentación (CIAL, CSIC-UAM), Nicolás Cabrera, Madrid, Spain.
Allergy Asthma Immunol Res. 2016 May;8(3):239-45. doi: 10.4168/aair.2016.8.3.239.
Two mouse strains, BALB/c and C3H/HeOuJ, broadly used in the field of food allergy, were compared for the evaluation of the allergenic potential of ovalbumin (OVA).
Sensitization was made by administering 2 different OVA doses (1 and 5 mg), with cholera toxin as Th2-polarizing adjuvant. Antibody levels, severity of anaphylaxis, and Th1 and Th2 responses induced by the allergen were assessed. In addition, because the mice selected had functional toll-like receptor 4, the influence of contamination with lipopolysaccharide (LPS) on the immunostimulating capacity of OVA on spleen cells was also evaluated.
Both strains exhibited similar susceptibility to OVA sensitization. The 2 protein doses generated similar OVA-specific IgE and IgG1 levels in both strains, whereas C3H/HeOuJ mice produced significantly more IgG2a. Oral challenge provoked more severe manifestations in C3H/HeOuJ mice as indicated by the drop in body temperature and the severity of the anaphylactic scores. Stimulation of splenocytes with OVA led to significantly higher levels of Th2 and Th1 cytokines in BALB/c, and these were less affected by protein contamination with LPS.
The antibody and cytokine levels induced by OVA in BALB/c mice and the observation that BALB/c spleen cell cultures were more resistant than those of C3H/HeOuJ mice to the stimulus of LPS make this strain prone to exhibit Th2-mediated food allergic reactions and very adequate for the study of the features of OVA that make it allergenic.
比较在食物过敏领域广泛使用的两种小鼠品系BALB/c和C3H/HeOuJ，以评估卵清蛋白（OVA）的致敏潜力。
通过给予2种不同剂量的OVA（1和5毫克）进行致敏，使用霍乱毒素作为Th2极化佐剂。评估抗体水平、过敏反应的严重程度以及过敏原诱导的Th1和Th2反应。此外，由于所选小鼠具有功能性Toll样受体4，还评估了脂多糖（LPS）污染对OVA刺激脾细胞免疫刺激能力的影响。
两种品系对OVA致敏表现出相似的易感性。两种蛋白剂量在两种品系中产生相似的OVA特异性IgE和IgG1水平，而C3H/HeOuJ小鼠产生的IgG2a明显更多。口服激发在C3H/HeOuJ小鼠中引发更严重的表现，体温下降和过敏评分的严重程度表明了这一点。用OVA刺激脾细胞导致BALB/c中Th2和Th1细胞因子水平显著更高，并且这些受LPS蛋白质污染的影响较小。
OVA在BALB/c小鼠中诱导的抗体和细胞因子水平，以及BALB/c脾细胞培养物比C3H/HeOuJ小鼠的脾细胞培养物对LPS刺激更具抗性的观察结果，使得该品系易于表现出Th2介导的食物过敏反应，并且非常适合研究使OVA具有致敏性的特征。
