Allergy Group, Department of Nutrition and Health, Nestle Research Center, Lausanne, Switzerland.
Clin Exp Allergy. 2010 Jun;40(6):942-50. doi: 10.1111/j.1365-2222.2010.03460.x. Epub 2010 Feb 17.
The immune system may be modulated with nutrition to prevent the development or to treat the symptoms of allergy. Among other foods, consumption of apples has been linked to reduced incidence of atopic dermatitis and respiratory allergy.
We evaluated the efficacy and mechanisms of a polyphenol-enriched apple extract in reducing symptoms of food allergy.
In a model of food allergy to ovalbumin (OVA), BALB/c mice were fed with an apple extract either during sensitization or just before the challenge. After the challenge, allergic symptoms were scored, OVA-specific serum immunoglobulins were determined by ELISA, cytokine production by mesenteric lymph node (MLN) cells was measured by a multiplex assay and gene expression profiles in the intestine were addressed using quantitative real-time PCR.
Consumption of the apple extract reduced symptoms of food allergy upon challenge. This was paralleled by reduced levels of intestinal mast cell protease, diminished cytokine secretion by MLN cells and reduced local intestinal mRNA expression of various T-helper type-2 associated and pro-inflammatory genes. Mechanistic studies suggested decrease of mediator release by effector cells and reduction of allergenicity by protein-polyphenol interaction as potential mechanisms responsible for protection.
Polyphenol-enriched apple extract can attenuate food allergy symptoms in sensitized mice via two distinct possible mechanisms.
免疫系统可以通过营养调节来预防过敏的发生或治疗过敏症状。在其他食物中，苹果的消费与特应性皮炎和呼吸道过敏发病率的降低有关。
我们评估了富含多酚的苹果提取物在减轻食物过敏症状方面的功效和机制。
在卵清蛋白（OVA）食物过敏模型中，BALB/c 小鼠在致敏期间或仅在挑战前用苹果提取物喂养。在挑战后，评估过敏症状，通过 ELISA 测定 OVA 特异性血清免疫球蛋白，通过多重分析测定肠系膜淋巴结（MLN）细胞的细胞因子产生，并用定量实时 PCR 研究肠道中的基因表达谱。
食用苹果提取物可减少过敏挑战时的食物过敏症状。这与肠道肥大细胞蛋白酶水平降低、MLN 细胞细胞因子分泌减少以及各种 T 辅助型 2 相关和促炎基因的局部肠道 mRNA 表达降低相平行。机制研究表明，效应细胞释放介质减少和蛋白质-多酚相互作用降低过敏原性可能是保护的潜在机制。
富含多酚的苹果提取物可通过两种不同的可能机制减轻致敏小鼠的食物过敏症状。
