Moretti Elena, Mazzi Lucia, Bonechi Claudia, Salvatici Maria Cristina, Iacoponi Francesca, Rossi Claudio, Collodel Giulia
Department of Molecular and Developmental Medicine, Policlinico Le Scotte, Viale Bracci 14, 53100 Siena, Italy.
Department of Molecular and Developmental Medicine, Policlinico Le Scotte, Viale Bracci 14, 53100 Siena, Italy.
Reprod Toxicol. 2016 Apr;60:140-7. doi: 10.1016/j.reprotox.2016.02.012. Epub 2016 Feb 24.
A strategy to circumvent the poor polyphenols bioavailability is to load these compounds into liposomes. We evaluated the in vitro effects of quercetin (Q) and Q-loaded liposomes (QLL, 30, 50, 100μM) on motility, viability and chromatin integrity of swim-up selected human sperm. Antioxidant power was assayed against tert-butylhydroperoxide induced lipid peroxidation (LPO) using C11-BODIPY581/591 fluorescent probe and transmission electron microscopy. QLL showed decreased toxicity for sperm motility and viability and increased DNA damage compared to Q. The percentage of sperm with fluorescence, marker of LPO, was decreased in samples incubated with Q vs QLL (P<0.001). The ultrastructure of acrosomes and membranes was preserved with Q 30/100μM, whereas QLL did not prevent membrane injury. Q alone appeared more effective than Q incorporated into liposomes; however liposomes could be considered as carriers that may convey different compounds inside sperm; they may therefore represent a field of research rich of many applications.
一种规避多酚生物利用度低的策略是将这些化合物载入脂质体。我们评估了槲皮素（Q）和载有槲皮素的脂质体（QLL，30、50、100μM）对上浮法筛选的人类精子活力、生存力和染色质完整性的体外作用。使用C11 - BODIPY581/591荧光探针和透射电子显微镜，针对叔丁基过氧化氢诱导的脂质过氧化（LPO）测定抗氧化能力。与Q相比，QLL对精子活力和生存力的毒性降低，而DNA损伤增加。与QLL孵育的样本相比，用Q孵育的样本中具有荧光（LPO标志物）的精子百分比降低（P<0.001）。30/100μM的Q可保留顶体和膜的超微结构，而QLL不能防止膜损伤。单独的Q似乎比载入脂质体的Q更有效；然而，脂质体可被视为能够将不同化合物输送到精子内部的载体；因此，它们可能代表一个具有许多应用的丰富研究领域。
