Zeng Chaoting, Shang Wenting, Wang Kun, Chi Chongwei, Jia Xiaohua, Fang Cheng, Yang Du, Ye Jinzuo, Fang Chihua, Tian Jie
Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China.
Key Laboratory of Molecular Imaging, Institute of Automation, Chinese Academy of Sciences, Beijing 100190, China.
Sci Rep. 2016 Feb 29;6:21959. doi: 10.1038/srep21959.
Difficulties in the highly sensitive detection of tumour microfoci represent a critical obstacle toward improved surgical intervention in liver cancer. Conventional preoperative imaging methods and surgeons' subjective experience are limited by their inability to effectively detect tumour lesions measuring less than 2 mm; however, intraoperative fluorescence molecular imaging may overcome this limitation. Here, we synthesised an arginine-glycine-aspartic acid (RGD)-conjugated mesoporous silica nanoparticle (MSN) highly loaded with indocyanine green (ICG) dye that could accurately delineate liver cancer margins and provide excellent tumour-to-normal tissue contrast intraoperatively. The increased ICG loading capacity and tumour specificity enabled the identification of residual microtumours and satellite lesions measuring less than 1 mm in living mice. Histological analysis validated the sensitivity and accuracy of this approach. We believe this technique utilising a new fluorescent nanoprobe with intraoperative optical imaging may offer a more sensitive and accurate method for liver cancer resection guidance, resulting in better surgical outcomes.
肿瘤微病灶的高灵敏度检测困难是改善肝癌手术干预的关键障碍。传统的术前成像方法和外科医生的主观经验受限于无法有效检测小于2毫米的肿瘤病变；然而，术中荧光分子成像可能克服这一限制。在此，我们合成了一种高负载吲哚菁绿（ICG）染料的精氨酸-甘氨酸-天冬氨酸（RGD）共轭介孔二氧化硅纳米颗粒（MSN），其可在术中准确勾勒出肝癌边缘并提供出色的肿瘤与正常组织对比度。增加的ICG负载能力和肿瘤特异性能够识别活体小鼠中小于1毫米的残留微肿瘤和卫星病灶。组织学分析验证了该方法的灵敏度和准确性。我们相信，这种利用术中光学成像的新型荧光纳米探针技术可能为肝癌切除指导提供一种更灵敏、准确的方法，从而带来更好的手术效果。
