Peña-Fleitas M T, Gallardo M, Thompson R B, Farneselli M, Padilla F M
Department of AgronomyUniversity of AlmeriaAlmeríaSpain; BITAL Research Centre for Agricultural and Food BiotechnologyUniversity of AlmeriaAlmeríaSpain.
Department of Agricultural Food and Environmental Sciences University of Perugia Perugia Italy.
Ann Appl Biol. 2015 Nov;167(3):387-405. doi: 10.1111/aab.12235. Epub 2015 Jul 2.
Evaluation of crop N status will assist optimal N management of intensive vegetable production. Simple procedures for monitoring crop N status such as petiole sap [NO -N], leaf N content and soil solution [NO ] were evaluated with indeterminate tomato and muskmelon. Their sensitivity to assess crop N status throughout each crop was evaluated using linear regression analysis against nitrogen nutrition index (NNI) and crop N content. NNI is the ratio between the actual and the critical crop N contents (critical N content is the minimum N content necessary to achieve maximum growth), and is an established indicator of crop N status. Nutrient solutions with four different N concentrations (treatments N1-N4) were applied throughout each crop. Average applied N concentrations were 1, 5, 13 and 22 mmol L in tomato, and 2, 7, 13 and 21 mmol L in muskmelon. Respective rates of N were 23, 147, 421 and 672 kg N ha in tomato, and 28, 124, 245 and 380 kg N ha in muskmelon. For each N treatment in each crop, petiole sap [NO -N] was relatively constant throughout the crop. During both crops, there were very significant (P < 0.001) linear relationships between both petiole sap [NO -N] and leaf N content with NNI and with crop N content. In indeterminate tomato, petiole sap [NO -N] was very strongly linearly related to NNI (R = 0.88-0.95, P < 0.001) with very similar slope and intercept values on all dates. Very similar relationships were obtained from published data of processing tomato. A single linear regression (R = 0.77, P < 0.001) described the relationship between sap [NO -N] and NNI for both indeterminate and processing tomato, each grown under very different conditions. A single sap [NO -N] sufficiency value of 1050 mg N L was subsequently derived for optimal crop N nutrition (at NNI = 1) of tomato grown under different conditions. In muskmelon, petiole sap [NO -N] was strongly linearly related to NNI (R = 0.75 - 0.88, P < 0.001) with very similar slope and intercept values for much of the crop (44-72 DAT, days after transplanting). A single linear relationship between sap [NO -N] and NNI (R = 0.77, P < 0.001) was derived for this period, but sap sufficiency values could not be derived for muskmelon as NNI values were >1. Relationships between petiole sap [NO -N] with crop N content, and leaf N content with both NNI and crop N content had variable slopes and intercept values during the indeterminate tomato and the muskmelon crops. Soil solution [NO ] in the root zone was not a sensitive indicator of crop N status. Of the three systems examined for monitoring crop/soil N status, petiole sap [NO -N] is suggested to be the most useful because of its sensitivity to crop N status and because it can be rapidly analysed on the farm.
评估作物氮素状况有助于集约化蔬菜生产的优化氮肥管理。采用无限生长型番茄和甜瓜对监测作物氮素状况的简单方法进行了评估,如叶柄汁液[NO₃-N]、叶片氮含量和土壤溶液[NO₃]。通过与氮营养指数(NNI)和作物氮含量进行线性回归分析,评估了它们在整个作物生长过程中对评估作物氮素状况的敏感性。NNI是实际作物氮含量与临界作物氮含量之比(临界氮含量是实现最大生长所需的最低氮含量),是已确立的作物氮素状况指标。在每种作物的整个生长过程中,施用了四种不同氮浓度的营养液(处理N1 - N4)。番茄中平均施用的氮浓度分别为1、5、13和22 mmol·L,甜瓜中分别为2、7、13和21 mmol·L。番茄中各自的施氮量分别为23、147、421和672 kg N·ha,甜瓜中分别为28、124、245和380 kg N·ha。对于每种作物的每个氮处理,叶柄汁液[NO₃-N]在整个作物生长过程中相对稳定。在两种作物生长期间,叶柄汁液[NO₃-N]和叶片氮含量与NNI以及与作物氮含量之间均存在极显著(P < 0.001)的线性关系。在无限生长型番茄中,叶柄汁液[NO₃-N]与NNI呈非常强的线性关系(R = 0.88 - 0.95,P < 0.001),在所有日期的斜率和截距值非常相似。从加工番茄的已发表数据中也获得了非常相似的关系。一个单一的线性回归(R = 0.77,P < 0.001)描述了无限生长型番茄和加工番茄在非常不同的生长条件下,汁液[NO₃-N]与NNI之间的关系。随后得出了一个单一的汁液[NO₃-N]充足值1050 mg N·L,用于不同条件下生长的番茄的最佳作物氮营养(NNI = 1)。在甜瓜中,叶柄汁液[NO₃-N]与NNI呈强线性关系(R = 0.75 - 0.88,P < 0.001)在作物生长的大部分时间(移栽后天数44 - 72天)斜率和截距值非常相似。在此期间得出了汁液[NO₃-N]与NNI之间的单一线性关系(R = 0.77,P < 0.001),但由于甜瓜的NNI值>1,无法得出其汁液充足值。在无限生长型番茄和甜瓜作物生长过程中,叶柄汁液[NO₃-N]与作物氮含量之间以及叶片氮含量与NNI和作物氮含量之间的关系具有可变的斜率和截距值。根区土壤溶液[NO₃]不是作物氮素状况的敏感指标。在所研究的三种监测作物/土壤氮素状况的系统中,叶柄汁液[NO₃-N]因其对作物氮素状况的敏感性以及可在农场快速分析而被认为是最有用的。