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具有不完全反硝化途径的解淀粉嗜热栖热菌模式菌株YIM 77409(T)的高质量基因组草图序列

High-quality draft genome sequence of the Thermus amyloliquefaciens type strain YIM 77409(T) with an incomplete denitrification pathway.

作者信息

Zhou En-Min, Murugapiran Senthil K, Mefferd Chrisabelle C, Liu Lan, Xian Wen-Dong, Yin Yi-Rui, Ming Hong, Yu Tian-Tian, Huntemann Marcel, Clum Alicia, Pillay Manoj, Palaniappan Krishnaveni, Varghese Neha, Mikhailova Natalia, Stamatis Dimitrios, Reddy T B K, Ngan Chew Yee, Daum Chris, Shapiro Nicole, Markowitz Victor, Ivanova Natalia, Spunde Alexander, Kyrpides Nikos, Woyke Tanja, Li Wen-Jun, Hedlund Brian P

机构信息

Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091 People's Republic of China ; School of Life Sciences, University of Nevada Las Vegas, Las Vegas, NV USA.

School of Life Sciences, University of Nevada Las Vegas, Las Vegas, NV USA.

出版信息

Stand Genomic Sci. 2016 Feb 27;11:20. doi: 10.1186/s40793-016-0140-3. eCollection 2016.

Abstract

Thermus amyloliquefaciens type strain YIM 77409(T) is a thermophilic, Gram-negative, non-motile and rod-shaped bacterium isolated from Niujie Hot Spring in Eryuan County, Yunnan Province, southwest China. In the present study we describe the features of strain YIM 77409(T) together with its genome sequence and annotation. The genome is 2,160,855 bp long and consists of 6 scaffolds with 67.4 % average GC content. A total of 2,313 genes were predicted, comprising 2,257 protein-coding and 56 RNA genes. The genome is predicted to encode a complete glycolysis, pentose phosphate pathway, and tricarboxylic acid cycle. Additionally, a large number of transporters and enzymes for heterotrophy highlight the broad heterotrophic lifestyle of this organism. A denitrification gene cluster included genes predicted to encode enzymes for the sequential reduction of nitrate to nitrous oxide, consistent with the incomplete denitrification phenotype of this strain.

摘要

嗜热栖热放线菌模式菌株YIM 77409(T)是一种嗜热、革兰氏阴性、无运动性的杆状细菌,从中国西南部云南省洱源县牛街温泉中分离得到。在本研究中,我们描述了菌株YIM 77409(T)的特征及其基因组序列和注释。该基因组长度为2,160,855 bp,由6个支架组成,平均GC含量为67.4%。共预测到2,313个基因,包括2,257个蛋白质编码基因和56个RNA基因。预计该基因组编码完整的糖酵解、磷酸戊糖途径和三羧酸循环。此外,大量用于异养的转运蛋白和酶突出了该生物体广泛的异养生活方式。一个反硝化基因簇包含预测编码将硝酸盐顺序还原为一氧化二氮的酶的基因,这与该菌株的不完全反硝化表型一致。

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