Mahittikorn Aongart, Kittichathanakul Thanuntorn, To-Im Jongdee, Nacapunchai Duangporn
Department of Protozoology (A.M.), Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Department of Clinical Chemistry (T.K., D.N.), Faculty of Medical Technology, Mahidol University, Nakhon Pathom, Thailand; and Faculty of Environment and Resource Studies (J.T.-I.), Mahidol University, Nakhon Pathom, Thailand.
Eye Contact Lens. 2017 Mar;43(2):81-88. doi: 10.1097/ICL.0000000000000246.
To assess the general knowledge, behavior, and presence of potentially pathogenic amoebae in cosmetic contact lens (CCL) wearers.
One hundred CCL asymptomatic wearers were randomly selected. A questionnaire regarding their lens use, and a pair of their CCL was obtained. Identification of free-living amoeba (FLA) strains was based on morphological diagnosis, enflagellation tests (for non-Acanthamoeba strains), and sequencing of the small-subunit rRNA gene fragments.
Most (92%) of the participants surveyed were women, and the average age of the participants was 21.5±0.2 years. The CCL wearers generally showed a moderate (47%) or good (35%) level of knowledge, and good (51%) or excellent (40%) use of CCL. Two CCL samples were positive for Acanthamoeba genotype T3 or Vahlkampfia. The Acanthamoeba-contaminated CCL was from a wearer who used saline for treating lenses, and the Vahlkampfia-contaminated CCL was from a wearer who used CCL while swimming.
This is the first report of the presence of potentially pathogenic FLA in used CCL from asymptomatic wearers in Thailand. Although there was satisfactory knowledge and practice of lens care use, the public should be aware of CCL contaminated with potentially pathogenic FLA that can directly or indirectly cause keratitis.
评估佩戴软性角膜接触镜（CCL）者的一般知识、行为以及潜在致病性阿米巴的存在情况。
随机选取100名无症状的CCL佩戴者。获取一份关于他们镜片使用情况的问卷以及他们的一副CCL。自由生活阿米巴（FLA）菌株的鉴定基于形态学诊断、鞭毛试验（针对非棘阿米巴菌株）以及小亚基rRNA基因片段测序。
参与调查的大多数（92%）为女性，参与者的平均年龄为21.5±0.2岁。CCL佩戴者总体上知识水平中等（47%）或良好（35%），CCL使用情况良好（51%）或优秀（40%）。两份CCL样本棘阿米巴基因型T3或瓦氏耐格里属呈阳性。被棘阿米巴污染的CCL来自一名使用生理盐水护理镜片的佩戴者，被瓦氏耐格里属污染的CCL来自一名游泳时佩戴CCL的佩戴者。
这是泰国首次报道无症状佩戴者使用过的CCL中存在潜在致病性FLA。尽管在镜片护理使用方面有令人满意的知识和实践，但公众应意识到被潜在致病性FLA污染的CCL可能直接或间接导致角膜炎。
