通过离子淌度-质谱法区分糖肽上的N-乙酰神经氨酸连接异构体
Distinguishing N-acetylneuraminic acid linkage isomers on glycopeptides by ion mobility-mass spectrometry.
作者信息
Hinneburg H, Hofmann J, Struwe W B, Thader A, Altmann F, Varón Silva D, Seeberger P H, Pagel K, Kolarich D
机构信息
Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
Department of Biology, Chemistry, Pharmacy, Freie Universität Berlin, 14195 Berlin, Germany.
出版信息
Chem Commun (Camb). 2016 Mar 21;52(23):4381-4. doi: 10.1039/c6cc01114d.
Differentiating the structure of isobaric glycopeptides represents a major challenge for mass spectrometry-based characterisation techniques. Here we show that the regiochemistry of the most common N-acetylneuraminic acid linkages of N-glycans can be identified in a site-specific manner from individual glycopeptides using ion mobility-mass spectrometry analysis of diagnostic fragment ions.
区分等压糖肽的结构是基于质谱的表征技术面临的一项重大挑战。在此，我们表明，使用诊断性碎片离子的离子淌度-质谱分析，可从单个糖肽中以位点特异性方式鉴定N-聚糖最常见的N-乙酰神经氨酸连接的区域化学。
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