Su H N, Xu Y Y, Wang X, Zhang K Q, Li G H
Laboratory for Conservation and Utilization of Bio-resource, Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming, China.
Angang general hospital, Anshan, China.
Lett Appl Microbiol. 2016 Apr;62(4):349-53. doi: 10.1111/lam.12557.
A total of 11 bacterial strains were assayed for bacteria-induced trap formation in the nematode-trapping fungus Arthrobotrys oligospora YMF1·01883 with two-compartmented Petri dish. These strains were identified on the basis of their 16S rRNA gene sequences. Volatile organic compounds (VOCs) of eight isolates were extracted using solid-phase micro-extraction (SPME) and their structures were identified based on gas chromatography-mass spectrometry (GC-MS). At the same time, all isolates were used for quantitative measurement of ammonia by the indophenol blue method. The effects of pure commercial compounds on inducement of trap formation in A. oligospora were tested. Taken together, results demonstrated that the predominant bacterial volatile compound inducing trap formation was ammonia. Meanwhile, ammonia also played a role in other nematode-trapping fungi, including Arthrobotrys guizhouensis YMF1·00014, producing adhesive nets; Dactylellina phymatopaga YMF1·01474, producing adhesive knobs; Dactylellina cionopaga YMF1·01472, producing adhesive columns and Drechslerella brochopaga YMF1·01829, producing constricting rings.
总共11株细菌菌株通过双隔培养皿，在捕食线虫真菌少孢节丛孢菌YMF1·01883中检测其诱导捕食器形成的能力。这些菌株基于其16S rRNA基因序列进行鉴定。使用固相微萃取（SPME）提取8株分离菌的挥发性有机化合物（VOCs），并基于气相色谱 - 质谱联用（GC - MS）鉴定其结构。同时，所有分离菌采用靛酚蓝法进行氨的定量测定。测试了纯商业化合物对少孢节丛孢菌捕食器形成诱导的影响。综合来看，结果表明诱导捕食器形成的主要细菌挥发性化合物是氨。同时，氨在其他捕食线虫真菌中也发挥作用，包括产生粘性网的贵州节丛孢菌YMF1·00014、产生粘性瘤的肿孢隔指孢菌YMF1·01474、产生粘性柱的柱隔指孢菌YMF1·01472以及产生收缩环的短柄德氏霉YMF1·01829。
