Pathogenicity and Volatile Nematicidal Metabolites from against .

Plant parasitic nematodes, especially parasitic root-knot nematodes, are one of the most destructive plant pathogens worldwide. The control of plant root-knot nematodes is extremely challenging. is a type of nematode-trapping fungi (NTF), which produces three-dimensional adhesive networks to trap nematodes. In this study, the pathogenicity and volatile organic compounds (VOCs) of the NTF against the plant root-knot nematode, , were investigated. The predatory process of trapping was observed using scanning electron microscopy. Gas chromatography-mass spectrometry analysis of the VOCs from led to the identification of 52 metabolites, of which 11 main compounds were tested individually for their activity against . Three compounds, cyclohexanamine, cyclohexanone, and cyclohexanol, were toxic to . Furthermore, these three VOCs inhibited egg hatching of . Cyclohexanamine showed the highest nematicidal activity, which can cause 97.93% mortality of at 8.71 µM within 12 h. The number of hatched juveniles per egg mass after 3 days was just 8.44 when treated with 26.14 µM cyclohexanamine. This study is the first to demonstrate the nematicidal activity of VOCs produced by against , which indicates that has the potential to biocontrol plant root-knot nematodes.