a Departamento de Neuroquímica , Instituto Nacional de Neurología y Neurocirugía "Manuel Velasco Suárez" , Insurgentes Sur #3877, Col. La Fama, Tlalpan, 14269 México D.F. , México.
b Laboratorio de Investigación de Bioquímica y Biofísica Computacional , Escuela Nacional de Medicina y de Homeopatía del IPN , Guillermo Massieu H. #239. La Escalera, Gustavo A. Madero, 07320 México D.F. , México.
Nutr Neurosci. 2017 Sep;20(7):388-395. doi: 10.1080/1028415X.2016.1147683. Epub 2016 Feb 29.
Essential fatty acids have an important effect on oxidative stress-related diseases. The Huntington's disease (HD) is a hereditary neurologic disorder in which oxidative stress caused by free radicals is an important damage mechanism. The HD experimental model induced by quinolinic acid (QUIN) has been widely used to evaluate therapeutic effects of antioxidant compounds. The aim of this study was to test whether the fatty acid content in olive- or fish-oil-rich diet prevents against QUIN-related oxidative damage in rats. Rats were fed during 20 days with an olive- or a fish-oil-rich diet (15% w/w). Posterior to diet period, rats were striatally microinjected with QUIN (240 nmol/µl) or saline solution. Then, we evaluated the neurological damage, oxidative status, and gamma isoform of the peroxisome proliferator-activated receptor (PPARγ) expression. Results showed that fatty acid-rich diet, mainly by fish oil, reduced circling behavior, prevented the fall in GABA levels, increased PPARγ expression, and prevented oxidative damage in striatal tissue. In addition none of the enriched diets exerted changes neither on triglycerides or cholesterol blood levels, nor or hepatic function. This study suggests that olive- and fish-oil-rich diets exert neuroprotective effects.
必需脂肪酸对与氧化应激相关的疾病有重要影响。亨廷顿病（HD）是一种遗传性神经疾病，其中自由基引起的氧化应激是一种重要的损伤机制。喹啉酸（QUIN）诱导的 HD 实验模型已广泛用于评估抗氧化化合物的治疗效果。本研究旨在测试富含橄榄油或鱼油的饮食中的脂肪酸含量是否可以预防 QUIN 引起的大鼠氧化损伤。大鼠在 20 天内用富含橄榄油或鱼油的饮食（15%w/w）喂养。饮食期结束后，大鼠纹状体中被微注射 QUIN（240nmol/µl）或生理盐水。然后，我们评估了神经损伤、氧化状态和过氧化物酶体增殖物激活受体（PPARγ）的γ同工型表达。结果表明，富含脂肪酸的饮食，主要是鱼油，可以减少转圈行为，防止 GABA 水平下降，增加 PPARγ 的表达，并防止纹状体组织的氧化损伤。此外，这些富含饮食均未改变甘油三酯或胆固醇的血液水平，也未改变肝功能。本研究表明，富含橄榄油和鱼油的饮食具有神经保护作用。
