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ADE3中的突变会降低全能抑制因子sup45-2的效率。

Mutations in ADE3 reduce the efficiency of the omnipotent suppressor sup45-2.

作者信息

Song J M, Liebman S W

机构信息

Department of Biological Sciences, University of Illinois, Chicago 60680.

出版信息

Curr Genet. 1989 Dec;16(5-6):315-21. doi: 10.1007/BF00340709.

DOI:10.1007/BF00340709
PMID:2692849
Abstract

Mutations in a known yeast gene, ADE3, were shown to act as an antisuppressor, reducing the efficiency of the omnipotent suppressor, sup45-2. The ADE3 locus encodes the trifunctional enzyme C1-tetrahydrofolate synthase, which is required for the biosynthesis of purines, thymidylate, methionine, histidine, pantothenic acid and formylmethionyl-tRNA(fMet. The role of this enzyme in translational fidelity had not previously been suspected.

摘要

已知酵母基因ADE3中的突变表现为反抑制因子，降低了全能抑制因子sup45-2的效率。ADE3基因座编码三功能酶C1-四氢叶酸合酶，该酶是嘌呤、胸苷酸、蛋氨酸、组氨酸、泛酸和甲酰甲硫氨酰-tRNA(fMet)生物合成所必需的。此前并未怀疑该酶在翻译保真度中的作用。

相似文献

1
Mutations in ADE3 reduce the efficiency of the omnipotent suppressor sup45-2.ADE3中的突变会降低全能抑制因子sup45-2的效率。
Curr Genet. 1989 Dec;16(5-6):315-21. doi: 10.1007/BF00340709.
2
Nucleotide sequence of the Saccharomyces cerevisiae ADE3 gene encoding C1-tetrahydrofolate synthase.编码C1-四氢叶酸合酶的酿酒酵母ADE3基因的核苷酸序列。
J Biol Chem. 1986 Apr 5;261(10):4629-37.
3
Regulation of expression of the ADE3 gene for yeast C1-tetrahydrofolate synthase, a trifunctional enzyme involved in one-carbon metabolism.酵母C1-四氢叶酸合成酶（一种参与一碳代谢的三功能酶）的ADE3基因表达调控。
J Biol Chem. 1985 Jan 25;260(2):1248-56.
4
Molecular genetic analysis of Saccharomyces cerevisiae C1-tetrahydrofolate synthase mutants reveals a noncatalytic function of the ADE3 gene product and an additional folate-dependent enzyme.酿酒酵母C1-四氢叶酸合酶突变体的分子遗传学分析揭示了ADE3基因产物的非催化功能以及另一种叶酸依赖性酶。
Mol Cell Biol. 1990 Nov;10(11):5679-87. doi: 10.1128/mcb.10.11.5679-5687.1990.
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A general method for generation and analysis of defined mutations in enzymes involved in a tetrahydrofolate-interconversion pathway.一种用于生成和分析参与四氢叶酸相互转化途径的酶中特定突变的通用方法。
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Rat C1-tetrahydrofolate synthase. cDNA isolation, tissue-specific levels of the mRNA, and expression of the protein in yeast.大鼠C1-四氢叶酸合酶。cDNA的分离、mRNA的组织特异性水平以及该蛋白在酵母中的表达。
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引用本文的文献

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Nonsense suppression in thymine-requiring strains of Escherichia coli is a consequence of altered folate metabolism.在需要胸腺嘧啶的大肠杆菌菌株中，无义抑制是叶酸代谢改变的结果。
Mol Gen Genet. 1993 Apr;238(1-2):218-24. doi: 10.1007/BF00279550.
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Function of yeast cytoplasmic C1-tetrahydrofolate synthase.酵母细胞质C1-四氢叶酸合成酶的功能。
Proc Natl Acad Sci U S A. 1993 Apr 1;90(7):2636-40. doi: 10.1073/pnas.90.7.2636.

本文引用的文献

1
4OS ribosomal protein from a Saccharomyces cerevisiae antisuppressor mutant exhibiting a unique 2D gel pattern.一个酵母反抑制突变体中 4OS 核糖体蛋白的独特二维凝胶图谱。
Curr Genet. 1981 Apr;3(1):27-9. doi: 10.1007/BF00419577.
2
Genetic and biochemical characterization of antisuppressor mutants in the yeast Saccharomyces cerevisiae.酵母酿酒酵母中反抑制突变体的遗传和生化特性。
Curr Genet. 1981 Dec;4(3):197-204. doi: 10.1007/BF00420499.
3
Purification of a complex catalyzing folate cofactor synthesis and transformylation in de novo purine biosynthesis.
在从头嘌呤生物合成中催化叶酸辅因子合成和转甲酰基反应的复合物的纯化。
J Biol Chem. 1980 Mar 10;255(5):1885-90.
4
An antisuppressor that acts on omnipotent suppressors in yeast.一种作用于酵母中全能抑制子的抗抑制子。
Genetics. 1980 May;95(1):49-61. doi: 10.1093/genetics/95.1.49.
5
Isolation and properties of an antisuppressor in Saccharomyces cerevisiae specific for an omnipotent suppressor.酿酒酵母中一种针对全能抑制子的反抑制子的分离与特性研究
J Bacteriol. 1980 Sep;143(3):1527-9. doi: 10.1128/jb.143.3.1527-1529.1980.
6
Formyl-methenyl-methylenetetrahydrofolate synthetase(combined) from yeast. Biochemical characterization of the protein from an ade3 mutant lacking the formyltetrahydrofolate synthetase function.来自酵母的甲酰-亚甲基-亚甲基四氢叶酸合成酶（组合型）。缺乏甲酰四氢叶酸合成酶功能的ade3突变体中该蛋白质的生化特性。
J Biol Chem. 1980 Mar 25;255(6):2569-77.
7
A comparison of yeast ribosomal protein gene DNA sequences.酵母核糖体蛋白基因DNA序列的比较
Nucleic Acids Res. 1984 Nov 26;12(22):8295-312. doi: 10.1093/nar/12.22.8295.
8
Transformation of intact yeast cells treated with alkali cations.经碱金属阳离子处理的完整酵母细胞的转化
J Bacteriol. 1983 Jan;153(1):163-8. doi: 10.1128/jb.153.1.163-168.1983.
9
Identification of a Ty insertion within the coding sequence of the S. cerevisiae URA3 gene.酿酒酵母URA3基因编码序列中一个Ty插入的鉴定。
Mol Gen Genet. 1984;193(3):557-60. doi: 10.1007/BF00382100.
10
Identification and expression of a cloned yeast heat shock gene.一个克隆的酵母热休克基因的鉴定与表达。
J Biol Chem. 1983 Feb 10;258(3):1908-13.