小鼠生长板发育分析
Analysis of Mouse Growth Plate Development.
作者信息
Mangiavini Laura, Merceron Christophe, Schipani Ernestina
机构信息
Department of Orthopaedic Surgery, Department of Medicine, Division of Endocrinology and Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, Michigan.
Inserm, UMRS 791-LIOAD, Centre for Osteoarticular and Dental Tissue Engineering, Group STEP 'Skeletal Tissue Engineering and Physiopathology', Nantes, France.
出版信息
Curr Protoc Mouse Biol. 2016 Mar 1;6(1):67-130. doi: 10.1002/9780470942390.mo150094.
To investigate skeletal development, pathophysiological mechanisms of cartilage and bone disease, and eventually assess innovative treatments, the mouse is a very important resource. During embryonic development, mesenchymal condensations are formed, and cells within these mesenchymal condensations either directly differentiate into osteoblasts and give origin to intramembranous bone, or differentiate into chondrocytes and form a cartilaginous anlage. The cartilaginous anlage or fetal growth plate is then replaced with bone. This process is also called endochondral bone development, and it is responsible for the generation of most of our skeleton. Here we discuss in detail the most common in vivo and in vitro techniques our laboratory is currently using for the analysis of the mouse fetal growth plate during development.
为了研究骨骼发育、软骨和骨疾病的病理生理机制，并最终评估创新疗法，小鼠是一种非常重要的资源。在胚胎发育过程中，会形成间充质凝聚物，这些间充质凝聚物中的细胞要么直接分化为成骨细胞并形成膜内成骨，要么分化为软骨细胞并形成软骨原基。然后软骨原基或胎儿生长板会被骨替代。这个过程也称为软骨内骨发育，它负责形成我们大部分的骨骼。在这里，我们将详细讨论我们实验室目前用于分析发育过程中小鼠胎儿生长板的最常见的体内和体外技术。
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