纳米颗粒表面对脂质体纳米颗粒高效液相色谱洗脱曲线的影响。
Effect of Nanoparticle Surface on the HPLC Elution Profile of Liposomal Nanoparticles.
作者信息
Itoh Naoki, Yamamoto Eiichi, Santa Tomofumi, Funatsu Takashi, Kato Masaru
机构信息
Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan.
DDS Research, Global Formulation Research Japan, Pharmaceutical Science and Technology, Core Function Unit, Eisai Product Creation Systems, Eisai Co., Ltd., 5-1-3 Tokodai, Tsukuba, Ibaraki, 300-2635, Japan.
出版信息
Pharm Res. 2016 Jun;33(6):1440-6. doi: 10.1007/s11095-016-1886-4. Epub 2016 Feb 29.
PURPOSE
Nanoparticles have been used in diverse areas, and even broader applications are expected in the future. Since surface modification can influence the configuration and toxicity of nanoparticles, a rapid screening method is important to ensure nanoparticle quality.
METHODS
We examined the effect of the nanoparticle surface morphology on the HPLC elution profile using two types of 100-nm liposomal nanoparticles (AmBisome(Ⓡ) and DOXIL(Ⓡ)).
RESULTS
These 100-nm-sized nanoparticles eluted before the holdup time (about 4 min), even when a column packed with particles with a relatively large pore size (30 nm) was used. The elution time of the nanoparticles increased with pegylation of the nanoparticles and protein adsorption to the nanoparticles; however, the nanoparticles still eluted before the holdup time.
CONCLUSIONS
The results of this study indicate that HPLC is a suitable tool for rapid evaluation of the surface of liposomal nanoparticles.
目的
纳米颗粒已应用于多个领域，预计未来还会有更广泛的应用。由于表面修饰会影响纳米颗粒的形态和毒性，因此快速筛选方法对于确保纳米颗粒质量很重要。
方法
我们使用两种100纳米的脂质体纳米颗粒（两性霉素B脂质体（安必素Ⓡ）和阿霉素脂质体（多柔比星脂质体Ⓡ））研究了纳米颗粒表面形态对高效液相色谱（HPLC）洗脱曲线的影响。
结果
即使使用填充有孔径相对较大（30纳米）颗粒的色谱柱，这些100纳米大小的纳米颗粒也在保留时间（约4分钟）之前洗脱。纳米颗粒的洗脱时间随着纳米颗粒的聚乙二醇化和蛋白质吸附到纳米颗粒上而增加；然而，纳米颗粒仍在保留时间之前洗脱。
结论
本研究结果表明，HPLC是快速评估脂质体纳米颗粒表面的合适工具。
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