One-stop Sharing Facility Center for Future Drug Discoveries, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
One-stop Sharing Facility Center for Future Drug Discoveries, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan; Laboratory of Protein Metabolism, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
J Pharm Biomed Anal. 2018 Jan 30;148:149-155. doi: 10.1016/j.jpba.2017.09.030. Epub 2017 Sep 25.
Much attention has been paid to nanoparticle-based drug delivery systems to selectively deliver drugs to target organs and tissues. In this study, AmBisome, an antimycotic nanomedicine containing amphotericin B, was chosen as a typical nanomedicine, and analyzed using dynamic light scattering (DLS), an easy method to measure size, and size distribution, atomic force microscopy (AFM) and high-performance liquid chromatography (HPLC). AFM allowed the analysis of shape, besides a detailed size and size distribution. HPLC is useful to quantify the released amount of amphotericin B as it succeeded in a rapid separation of AmBisome and free amphotericin B. The aggregation or collapse of AmBisome and release of amphotericin B occurred upon 75% methanol addition or dilution with buffer around pH 4. Therefore, it is important to analyze nanomedicines using multiple analytical methods, and to integrate them for the quality and quantity evaluation of nanomedicines.
人们非常关注基于纳米颗粒的药物传递系统，以将药物选择性递送到靶器官和组织。在本研究中，两性霉素 B 纳米药物 AmBisome 被选为一种典型的纳米药物，并使用动态光散射（DLS）、一种易于测量大小和粒径分布的方法、原子力显微镜（AFM）和高效液相色谱（HPLC）进行分析。AFM 除了可以进行详细的大小和粒径分布分析外，还可以分析形状。HPLC 可用于定量测定两性霉素 B 的释放量，因为它成功地实现了 AmBisome 和游离两性霉素 B 的快速分离。当添加 75%甲醇或用缓冲液稀释至 pH 值约为 4 时，AmBisome 会发生聚集或坍塌，并释放出两性霉素 B。因此，使用多种分析方法分析纳米药物，并将它们整合起来，对于纳米药物的质量和数量评估非常重要。
