Fortes-Gabriel Elsa, Carreira Teresa, Vieira Maria Luísa
Am J Trop Med Hyg. 2016 May 4;94(5):955-958. doi: 10.4269/ajtmh.15-0027. Epub 2016 Feb 29.
Rodents play an important role in the transmission of pathogenic Leptospira spp. However, in Angola, neither the natural reservoirs of these spirochetes nor leptospirosis diagnosis has been considered. Regarding this gap, we captured rodents in Luanda and Huambo provinces to identify circulating Leptospira spp. Rodent kidney tissue was cultured and DNA amplified and sequenced. Culture isolates were evaluated for pathogenic status and typing with rabbit antisera; polymerase chain reaction (PCR) and sequencing were also performed. A total of 37 rodents were captured: Rattus rattus (15, 40.5%), Rattus norvegicus (9, 24.3%), and Mus musculus (13, 35.2%). Leptospiral DNA was amplified in eight (21.6%) kidney samples. From the cultures, we obtained four (10.8%) Leptospira isolates belonging to the Icterohaemorrhagiae and Ballum serogroups of Leptospira interrogans and Leptospira borgpetersenii genospecies, respectively. This study provides information about circulating leptospires spread by rats and mice in Angola.
啮齿动物在致病性钩端螺旋体的传播中起重要作用。然而，在安哥拉，既未考虑这些螺旋体的自然宿主，也未对钩端螺旋体病进行诊断。针对这一空白，我们在罗安达省和万博省捕获啮齿动物，以鉴定循环存在的钩端螺旋体种类。对啮齿动物的肾脏组织进行培养，并对DNA进行扩增和测序。对培养分离株进行致病状态评估并用兔抗血清分型；同时进行聚合酶链反应（PCR）和测序。共捕获37只啮齿动物：黑家鼠（15只，占40.5%）、褐家鼠（9只，占24.3%）和小家鼠（13只，占35.2%）。在8份（21.6%）肾脏样本中扩增出钩端螺旋体DNA。从培养物中，我们分别获得了4株（10.8%）钩端螺旋体分离株，分别属于问号钩端螺旋体的出血性黄疸群和拜伦群以及博氏钩端螺旋体基因种。本研究提供了关于安哥拉大鼠和小鼠传播的循环钩端螺旋体的信息。
