Yauri Verónica, Castro-Sesquen Yagahira E, Verastegui Manuela, Angulo Noelia, Recuenco Fernando, Cabello Ines, Malaga Edith, Bern Caryn, Gavidia Cesar M, Gilman Robert H
Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima, Peru; Laboratorio de Investigación en Enfermedades Infecciosas, Universidad Peruana Cayetano Heredia, Lima, Peru; Department of Epidemiology and Biostatistics, University of California, San Francisco, San Francisco, California; Department of International Health, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland.
Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima, Peru; Laboratorio de Investigación en Enfermedades Infecciosas, Universidad Peruana Cayetano Heredia, Lima, Peru; Department of Epidemiology and Biostatistics, University of California, San Francisco, San Francisco, California; Department of International Health, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland
Am J Trop Med Hyg. 2016 May 4;94(5):1020-7. doi: 10.4269/ajtmh.15-0233. Epub 2016 Feb 29.
Pigs were infected with a Bolivian strain of Trypanosoma cruzi (genotype I) and evaluated up to 150 days postinoculation (dpi) to determine the use of pigs as an animal model of Chagas disease. Parasitemia was observed in the infected pigs during the acute phase (15-40 dpi). Anti-T. cruzi immunoglobulin M was detected during 15-75 dpi; high levels of anti-T. cruzi immunoglobulin G were detected in all infected pigs from 75 to 150 dpi. Parasitic DNA was observed by western blot (58%, 28/48) and polymerase chain reaction (27%, 13/48) in urine samples, and in the brain (75%, 3/4), spleen (50%, 2/4), and duodenum (25%, 1/4), but no parasitic DNA was found in the heart, colon, and kidney. Parasites were not observed microscopically in tissues samples, but mild inflammation, vasculitis, and congestion was observed in heart, brain, kidney, and spleen. This pig model was useful for the standardization of the urine test because of the higher volume that can be obtained as compared with other small animal models. However, further experiments are required to observe pathological changes characteristic of Chagas disease in humans.
猪感染了玻利维亚株克氏锥虫（基因型I），并在接种后长达150天（dpi）进行评估，以确定猪作为恰加斯病动物模型的用途。在急性期（15 - 40 dpi），感染猪中观察到了寄生虫血症。在15 - 75 dpi期间检测到抗克氏锥虫免疫球蛋白M；在75至150 dpi期间，所有感染猪中均检测到高水平的抗克氏锥虫免疫球蛋白G。通过蛋白质印迹法（58%，28/48）和聚合酶链反应（27%，13/48）在尿液样本以及脑（75%，3/4）、脾（50%，2/4）和十二指肠（25%，1/4）中观察到了寄生虫DNA，但在心脏、结肠和肾脏中未发现寄生虫DNA。在组织样本中未通过显微镜观察到寄生虫，但在心脏、脑、肾脏和脾脏中观察到了轻度炎症、血管炎和充血。由于与其他小动物模型相比可获得的尿量更大，该猪模型对于尿液检测的标准化很有用。然而，需要进一步的实验来观察人类恰加斯病的特征性病理变化。
