Schwertz C I, do Carmo G M, Bottari N B, da Silva E S, Gabriel M E, Lucca N J, Guarda N dos S, Moresco R N, Machado G, Morsch V M, Schetinger M R C, Stefani L M, Mendes R E, Da Silva A S
Laboratory of Veterinary Pathology, Instituto Federal Catarinense, Concórdia, Santa Catarina, Brazil.
Department of Biochemistry and Molecular Biology, Brazil.
J Comp Pathol. 2016 Feb-Apr;154(2-3):150-6. doi: 10.1016/j.jcpa.2016.01.009. Epub 2016 Feb 28.
The aim of this study was to evaluate the role of butyrylcholinesterase (BChE) (in the serum and pancreas), acetylcholinesterase (AChE) (in the whole blood and pancreas) and nitric oxide (NO) (in the serum and pancreas) in cattle infected naturally by Eurytrema coelomaticum. Fifty-one cattle were studied, including 33 infected by E. coelomaticum and 18 uninfected animals. Significantly greater AChE activity was found in the pancreas of infected animals (P <0.01); however, these cattle had lower AChE activity in whole blood. BChE activity was greater in the sera of infected animals (P = 0.05), but was less in pancreatic samples. NO levels were significantly higher in the sera (P <0.05) and pancreas (P <0.001) of infected cattle compared with uninfected animals. A positive correlation was found between AChE activity in the pancreas and parasite load, but there was negative correlation between pancreatic BChE activity and parasitic load. Expression of AChE, BChE and NO is therefore linked to the inflammation caused by E. coelomaticum in cattle.
本研究旨在评估丁酰胆碱酯酶（BChE）（血清和胰腺中）、乙酰胆碱酯酶（AChE）（全血和胰腺中）以及一氧化氮（NO）（血清和胰腺中）在自然感染腔阔盘吸虫的牛体内所起的作用。研究了51头牛，其中包括33头感染腔阔盘吸虫的牛和18头未感染的动物。在感染动物的胰腺中发现AChE活性显著更高（P<0.01）；然而，这些牛的全血中AChE活性较低。感染动物血清中的BChE活性更高（P=0.05），但胰腺样本中的BChE活性较低。与未感染动物相比，感染牛的血清（P<0.05）和胰腺（P<0.001）中的NO水平显著更高。胰腺中的AChE活性与寄生虫负荷之间呈正相关，但胰腺BChE活性与寄生虫负荷之间呈负相关。因此，AChE、BChE和NO的表达与腔阔盘吸虫在牛体内引起的炎症有关。
