Liu F, Wu X L, He Z Y, Xiang M C, He Y C, Liu X Z, Chen S J, Zeng W
Chongqing Academy of Chinese Materia Medica, Chongqing, China.
Chongqing Key Laboratory of Chinese Medicine Resources, Chongqing, China.
J Appl Microbiol. 2016 Jun;120(6):1605-15. doi: 10.1111/jam.13117. Epub 2016 Apr 4.
To investigate the nutritional requirements of the mycelial growth and pathogenesis-related enzymatic activity on Isaria farinosa and the nutritional effect of fungus on its host Hepialus larvae.
Sixteen carbon sources, 16 nitrogen sources and 9 vitamin combinations were tested. The nutritional conditions that significantly prevented I. farinosa pathogenesis were selected as feed additives for rearing Hepialus larvae. Citric acid significantly inhibited the growth of I. farinosa and the activity of three enzymes. l-histidine and l-aspartic acid significantly reduced the dry weights of mycelia and their protease and lipase activities. Vitamin combination that lacked VB 1 significantly increased the growth of I. farinosa and enhanced its chitinase and lipase activities. l-aspartic acid, VB 1 or a combination of them were beneficial for maintaining the larvae survival rate and decreasing the disease rate. The result provides new insight to develop a nutrition-based strategy to control fungal epidemics during insect rearing.
The ability of some specific nutrients to inhibit mycelial growth and enzymatic activity can prevent epidemics of fungal disease.
These results will aid in the development of nutrition-based strategies to control entomopathogenic fungal epidemics during the large-scale rearing of insects.
研究粉棒束孢菌丝生长和致病相关酶活性的营养需求，以及该真菌对其寄主蝙蝠蛾幼虫的营养效应。
测试了16种碳源、16种氮源和9种维生素组合。将显著抑制粉棒束孢致病的营养条件作为饲养蝙蝠蛾幼虫的饲料添加剂。柠檬酸显著抑制粉棒束孢的生长和三种酶的活性。L-组氨酸和L-天冬氨酸显著降低菌丝体干重及其蛋白酶和脂肪酶活性。缺乏维生素B1的维生素组合显著促进粉棒束孢的生长并增强其几丁质酶和脂肪酶活性。L-天冬氨酸、维生素B1或它们的组合有利于维持幼虫存活率并降低发病率。该结果为制定基于营养的策略以控制昆虫饲养过程中的真菌流行病提供了新见解。
某些特定营养物质抑制菌丝生长和酶活性的能力可预防真菌病害的流行。
这些结果将有助于制定基于营养的策略，以控制昆虫大规模饲养过程中的昆虫病原真菌流行病。
