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人工培养基和温度对蜜蜂幼虫病原体生长发育的影响

The Effect of Artificial Media and Temperature on the Growth and Development of the Honey Bee Brood Pathogen .

作者信息

Mráz Petr, Hýbl Marian, Kopecký Marek, Bohatá Andrea, Konopická Jana, Hoštičková Irena, Konvalina Petr, Šipoš Jan, Rost Michael, Čurn Vladislav

机构信息

Department of Genetics and Agricultural Biotechnology, Faculty of Agriculture, University of South Bohemia, Studentska 1668, 370 05 Ceske Budejovice, Czech Republic.

Department of Zoology, Fisheries, Hydrobiology and Apiculture, Faculty of AgriSciences, Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech Republic.

出版信息

Biology (Basel). 2021 May 12;10(5):431. doi: 10.3390/biology10050431.

DOI:10.3390/biology10050431
PMID:34066178
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8151570/
Abstract

is a causative agent of chalkbrood, which is one of the most widespread honey bee diseases. In our experiments, the influence of several artificial media and cultivation under different temperatures was evaluated. Concretely, the radial growth of separated mating types was measured, reproductive structures in a Neubauer hemocytometer chamber were counted simultaneously, and the morphometry of spore cysts and spore balls was assessed. The complex set of experiments determined suitable cultivation conditions. A specific pattern between reproductive structure size and temperature was found. The optimal temperature for both mating types was 30 °C. SDA and YGPSA media are suitable for fast mycelial growth. Moreover, the effect of bee brood on fungus growth and development in vitro was investigated by modification of culture medium. The newly modified medium PDA-BB4 was most effective for the production of the reproductive structures. The result suggests that honey bee brood provides necessary nutrients for proper fungus development during in vitro cultivation. As there is no registered therapeutic agent against chalkbrood in most countries, including the European Union, the assessment of growth and development in different conditions could help to understand fungus pathogenesis and thus control chalkbrood disease.

摘要

是白垩病的病原体，白垩病是最广泛传播的蜜蜂疾病之一。在我们的实验中，评估了几种人工培养基以及在不同温度下培养的影响。具体而言，测量了分离的交配型的径向生长，同时在血细胞计数板计数生殖结构，并评估了孢子囊肿和孢子球的形态学。这一系列复杂的实验确定了合适的培养条件。发现了生殖结构大小与温度之间的特定模式。两种交配型的最佳温度均为30℃。SDA和YGPSA培养基适合菌丝快速生长。此外，通过改良培养基研究了蜂幼虫对真菌体外生长和发育的影响。新改良的培养基PDA-BB4对生殖结构的产生最有效。结果表明，在体外培养过程中，蜜蜂幼虫为真菌的正常发育提供了必要的营养。由于在包括欧盟在内的大多数国家都没有针对白垩病的注册治疗剂，评估不同条件下的生长和发育有助于了解真菌发病机制，从而控制白垩病。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14a1/8151570/0bef01d03a7d/biology-10-00431-g010.jpg
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