Vadekeetil Anitha, Saini Hina, Chhibber Sanjay, Harjai Kusum
a Department of Microbiology , Panjab University , Chandigarh , India.
Biofouling. 2016;32(4):371-82. doi: 10.1080/08927014.2015.1137289.
The study investigated the in vitro, ex vivo and in vivo efficacy of ajoene and ciprofloxacin (CIP) alone and in combination against Pseudomonas aeruginosa biofilms and biofilm-associated murine acute pyelonephritis. The ajoene-CIP combination exhibited significant greater (p < 0.05) antimotility and biofilm inhibitory effects than those obtained when they were applied individually. The combined action of the agents resulted in a significant increase in serum sensitivity and phagocytic uptake and killing of P. aeruginosa (p < 0.001) compared to the untreated control. Mice groups treated with an ajoene (25 mg kg(-1)) and CIP (30 mg kg(-1) or 15 mg kg(-1)) combination showed a significantly (p < 0.001) reduced bacterial load in the kidney and bladder as compared to that of infected controls and mice treated with solo agents on the fifth day post-infection. The decreased levels of biomarkers and photomicrographs of the kidney tissue of the treated mice showed a reduced severity of damage. Hence, the study highlights the antivirulent and therapeutic efficacy of the ajoene-CIP combination at the minimal dosage of CIP.
该研究调查了大蒜素和环丙沙星（CIP）单独及联合使用对铜绿假单胞菌生物膜以及生物膜相关的小鼠急性肾盂肾炎的体外、离体和体内疗效。与单独使用时相比，大蒜素 - CIP组合表现出显著更强（p < 0.05）的抗运动性和生物膜抑制作用。与未处理的对照组相比，两种药物的联合作用导致血清敏感性显著提高，铜绿假单胞菌的吞噬摄取和杀灭能力显著增强（p < 0.001）。与感染对照组以及在感染后第5天接受单一药物治疗的小鼠相比，用大蒜素（25 mg kg⁻¹）和CIP（30 mg kg⁻¹或15 mg kg⁻¹）联合治疗的小鼠组肾脏和膀胱中的细菌载量显著降低（p < 0.001）。治疗小鼠肾脏组织的生物标志物水平降低以及显微照片显示损伤严重程度减轻。因此，该研究突出了大蒜素 - CIP组合在最低CIP剂量下的抗毒力和治疗效果。
