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生物传感器在滴水损失分析和糖酵解潜力评估中的应用。

The application of biosensors for drip loss analysis and glycolytic potential evaluation.

作者信息

Przybylski Wiesław, Sionek Barbara, Jaworska Danuta, Santé-Lhoutellier Véronique

机构信息

Department of Food Gastronomy and Food Hygiene, Faculty of Human Nutrition and Consumer Sciences, Warsaw University of Life Sciences, Nowoursynowska 159C str., 02-776 Warszawa, Poland.

Department of Food Gastronomy and Food Hygiene, Faculty of Human Nutrition and Consumer Sciences, Warsaw University of Life Sciences, Nowoursynowska 159C str., 02-776 Warszawa, Poland.

出版信息

Meat Sci. 2016 Jul;117:7-11. doi: 10.1016/j.meatsci.2016.02.025. Epub 2016 Feb 18.

Abstract

The aim of this study was to evaluate the relationship between glucose and lactate measured by biosensors in drip loss (strip method) with muscle glycolytic potential and their compounds. On the samples taken from Longissimus dorsi of 24 pigs (pure Neckar hybrid line) the following meat quality traits were determined: pH at 24h, meat color according to CIE L(⁎)a(⁎)b(⁎) system and drip loss. The highest correlations were found between glucose in drip loss and glycogen (r=0.84) or glycolytic potential (r=0.81) in muscle. A significant positive relationship between lactate measured in muscle by enzymatic method and by biosensor in drip loss was established (r=0.76). Glycogen, glucose, lactate and glycolytic potential with meat quality traits as ultimate pH, lightness, b(⁎) value and drip loss were significantly related. Results of multiple regression between glucose as well as lactate measured in muscle drip loss with muscle glycolytic potential showed the possibility of its prediction (r=0.87 with Pα≤0.001).

摘要

本研究的目的是评估通过生物传感器在滴水损失(试纸法)中测得的葡萄糖和乳酸与肌肉糖酵解潜力及其化合物之间的关系。对取自24头猪(纯种内卡杂交系)背最长肌的样本,测定了以下肉质性状:24小时时的pH值、根据CIE L(⁎)a(⁎)b(⁎)系统测定的肉色以及滴水损失。在滴水损失中的葡萄糖与肌肉中的糖原(r = 0.84)或糖酵解潜力(r = 0.81)之间发现了最高的相关性。通过酶法测定的肌肉中的乳酸与通过生物传感器在滴水损失中测得的乳酸之间建立了显著的正相关关系(r = 0.76)。糖原、葡萄糖、乳酸和糖酵解潜力与肉质性状如最终pH值、亮度、b(⁎)值和滴水损失显著相关。肌肉滴水损失中测得的葡萄糖以及乳酸与肌肉糖酵解潜力之间的多元回归结果表明了其预测的可能性(r = 0.87,Pα≤0.001)。

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