Tripathy Swapan K, Ranjan Rajesh, Dash Sasmita, Bastia Devraj Lenka Debendra N, Baisakh Bhabendra, Satpathy Pramod C, Sahu Simanchal
J Environ Biol. 2016 Jan;37(1):65-73.
A set of 36 scented rice mutants were developed through recurrent mutagenesis Pusa basmati-1, Pusa Sugandha-2 and Ketakijoha local with ethyl methane sulphonate (EMS), N-methyl N-nitro N-nitrosoguanidine (NG) and a combination of 0.4% EMS and 0.015% NG over two successive generations. ORM 256-8-10 and ORM 256-8-6 (mutants of PB-1) and ORM 228-1 (mutant of Pusa Sugandha 2) had shown significantly higher grain yield than Geetanjali (standard check), as well as, their respective parent varieties. The above test genotypes were analysed by 12 RAPD and 11 ISSR primers. RAPD and ISSR primers amplified 92 and 77 bands ranging from 4-15 and 4-12 bands per respective primer exhibiting higher level of polymorphism (86.95% and 94.80%). RAPD primer OU 1 and ISSR primer OUAT 7 produced maximum number of 15 (280-1830bp) and 12 polymorphic bands (420-1260bp), respectively. ISSR revealed higher polymorphic information content (PIC) values than RAPD primers indicating better allelic diversity. Resolving power revealed higher efficiency of RAPD primers. OU-1 with high GC content (80%) and two ISSR primers OUAT-7 (GC = 66.7%) and OUAT-15 (GC = 88.2%) produced higher number of polymorphic amplicons. ORM 228-1, Pusa Sugandha-2, ORM 256-8-10 and Ketakijoha were identified to be highly divergent genotypes based on RAPD and ISSR analyses. RAPD analysis revealed divergence of ORM 256-2 and ORM 256-8-12 while ORM 256-8-6 isolated from rest of the genotypes in case of ISSR. This could be attributed to genotype-specific RAPD and ISSR alleles. The above diverse genotypes with high yield identified in the present pursuit would enrich the basmati gene pool for further genetic improvement for grain quality and yield per se.
通过连续两代使用甲基磺酸乙酯（EMS）、N-甲基-N-硝基-N-亚硝基胍（NG）以及0.4% EMS和0.015% NG的组合对巴斯马蒂1号、普萨香稻2号和凯塔基乔哈本地品种进行反复诱变，培育出了一组36个香稻突变体。ORM 256-8-10和ORM 256-8-6（PB-1的突变体）以及ORM 228-1（普萨香稻2号的突变体）的籽粒产量显著高于吉檀迦利（标准对照）及其各自的亲本品种。使用12个RAPD引物和11个ISSR引物对上述测试基因型进行了分析。RAPD和ISSR引物分别扩增出92条和77条带，每个引物的条带数在4至15条和4至12条之间，表现出较高水平的多态性（分别为86.95%和94.80%）。RAPD引物OU 1和ISSR引物OUAT 7分别产生了最多数量的15条（280 - 1830bp）和12条多态性条带（420 - 1260bp）。ISSR显示出比RAPD引物更高的多态信息含量（PIC）值，表明等位基因多样性更好。分辨能力显示RAPD引物效率更高。具有高GC含量（80%）的OU-1以及两个ISSR引物OUAT-7（GC = 66.7%）和OUAT-15（GC = 88.2%）产生了更多数量的多态性扩增子。基于RAPD和ISSR分析，ORM 228-1、普萨香稻2号、ORM 256-8-10和凯塔基乔哈被鉴定为高度不同的基因型。RAPD分析揭示了ORM 256-2和ORM 256-8-12的差异，而在ISSR分析中，ORM 256-8-6与其他基因型分离。这可能归因于基因型特异性的RAPD和ISSR等位基因。在当前研究中鉴定出的上述具有高产的不同基因型将丰富巴斯马蒂基因库，以进一步进行谷物品质和产量本身的遗传改良。
