Florensa L, Besses C, Almarcha J, Lafuente R, Palou L, Pedro C, Sans-Sabrafen J, Woessner S
Hospital Creu Roja de Barcelona, Unidad de Hematología y Oncología, Spain.
Eur J Haematol. 1989 Nov;43(5):417-22. doi: 10.1111/j.1600-0609.1989.tb00329.x.
We studied the behaviour in culture of erythroid and megakaryocyte progenitor cells (BFU-E, CFU-MK) obtained from peripheral blood (PB) in 38 patients: 15 with essential thrombocythaemia, 3 with reactive thrombocytosis, 16 with polycythaemia vera and 4 with secondary polyglobulia. Clonal erythroid growth without added erythropoietin was observed in all patients with polycythaemia vera and in 5 out of 15 with essential thrombocythaemia, but in none of the patients with reactive thrombocytosis or secondary polyglobulia or in controls. When the CFU-MK were cultured without phytohaemagglutinin-stimulated medium (PHA-LCM), all patients with essential thrombocythaemia and 7 out of 16 with polycythaemia vera showed circulating CFU-MK but none of those with reactive thrombocytosis or secondary polyglobulia or controls did so. This study indicates that the growth in vitro of megakaryocytic and erythroid progenitors from such a readily available source as peripheral blood can be valuable in the diagnosis of certain borderline cases of thrombocytosis or erythrocytosis.
我们研究了从38例患者外周血（PB）中获取的红系和巨核系祖细胞（BFU-E、CFU-MK）在培养中的行为：15例原发性血小板增多症患者、3例反应性血小板增多症患者、16例真性红细胞增多症患者和4例继发性红细胞增多症患者。在所有真性红细胞增多症患者以及15例原发性血小板增多症患者中的5例中观察到了在未添加促红细胞生成素的情况下红系克隆生长，但反应性血小板增多症患者、继发性红细胞增多症患者或对照组患者均未出现这种情况。当在无植物血凝素刺激培养基（PHA-LCM）的情况下培养CFU-MK时，所有原发性血小板增多症患者以及16例真性红细胞增多症患者中的7例出现了循环CFU-MK，但反应性血小板增多症患者、继发性红细胞增多症患者或对照组患者均未出现这种情况。这项研究表明，从外周血这种易于获取的来源获取的巨核系和红系祖细胞的体外生长在某些血小板增多症或红细胞增多症的临界病例诊断中可能具有重要价值。
