Juvonen E, Partanen S, Ikkala E, Ruutu T
II Department of Medicine, Helsinki University Central Hospital, Finland.
Br J Haematol. 1988 Aug;69(4):441-4. doi: 10.1111/j.1365-2141.1988.tb02395.x.
Megakaryocytic colony formation by progenitor cells of 18 patients with polycythaemia vera, seven with secondary erythrocytosis and four with erythrocytosis of unexplained origin was studied in vitro by the methyl cellulose culture assay. Fourteen of the 18 patients with polycythaemia vera showed spontaneous megakaryocytic colony formation, i.e. colony growth with normal human plasma as the only source of colony stimulation. None of the patients with secondary erythrocytosis or erythrocytosis of unknown origin or of the normal controls grew colonies in the presence of normal human plasma only. When the plasma of a patient with aplastic anaemia was used instead of normal human plasma and phytohaemagglutinin stimulated leucocyte conditioned medium (PHA-LCM) was added to the culture medium, two of the patients with polycythaemia vera and one with secondary erythrocytosis formed slightly increased numbers of megakaryocytic colonies, while the rest of the patients showed normal colony formation. All of the patients with polycythaemia vera but none of those with secondary erythrocytosis or erythrocytosis of unknown origin showed spontaneous erythroid colony growth. The present study shows that most patients with polycythaemia vera form spontaneous megakaryocytic colonies in vitro. This phenomenon has recently also been demonstrated in essential thrombocythaemia and it is apparently analogous to spontaneous erythroid colony growth seen in all myeloproliferative disorders.
采用甲基纤维素培养试验对18例真性红细胞增多症患者、7例继发性红细胞增多症患者及4例不明原因红细胞增多症患者的祖细胞进行体外巨核细胞集落形成研究。18例真性红细胞增多症患者中有14例出现自发巨核细胞集落形成，即以正常人血浆作为唯一集落刺激源时集落生长。仅使用正常人血浆时，继发性红细胞增多症患者、不明原因红细胞增多症患者及正常对照者均未形成集落。当用再生障碍性贫血患者的血浆替代正常人血浆，并在培养基中添加植物血凝素刺激的白细胞条件培养基（PHA-LCM）时，2例真性红细胞增多症患者和1例继发性红细胞增多症患者形成的巨核细胞集落数量略有增加，而其余患者集落形成正常。所有真性红细胞增多症患者均出现自发红系集落生长，而继发性红细胞增多症患者及不明原因红细胞增多症患者均未出现。本研究表明，大多数真性红细胞增多症患者在体外可形成自发巨核细胞集落。最近在原发性血小板增多症中也证实了这一现象，且它显然类似于在所有骨髓增殖性疾病中所见的自发红系集落生长。
