Rolovic Z, Basara N, Gotic M, Sefer D, Bogdanovic A
Institute of Haematology, Clinical Centre of Serbia, Belgrade, Yugoslavia.
Br J Haematol. 1995 Jun;90(2):326-31. doi: 10.1111/j.1365-2141.1995.tb05153.x.
Spontaneous colony formation from bone marrow megakaryocyte progenitors (BMsCFU-Mk) was studied in 24 patients with essential thrombocythaemia (ET), 20 patients with reactive thrombocytosis (RT), 20 patients with polycthaemia rubra vera with thrombocytosis (PRVtr), 16 patients with chronic myeloid leukaemia with thrombocytosis (CMLtr) and 18 normal control subjects (C). The culture medium which was used in the methylcellulose assay in vitro contained 30% of plasma from a single patient with hereditary haemochromatosis. Remarkable BMsCFU-Mk growth was recorded in all patients with ET but in none with RT or in C. BMs-CFU-Mk were present in 11/20 patients with PRVtr and 7/16 patients with CMLtr. Spontaneous bone marrow erythroid progenitors (BMsBFU-E) were also determined in these patients. BMsBFU-E were found in 21/24 patients with ET and none in the patients with RT and C. All patients with PRVtr and one patient with CMLtr showed BMsBFU-E. We conclude that our implementation of the in vitro methylcellulose assay allows the BMsCFU-Mk to be used as an unequivocal test for discrimination between ET and RT which has not been shown in previously published studies. In addition, we present evidence that in 10 patients BMsCFU-Mk and/or BMsBFU-E growth in the test persisted after long-lasting haematological remission.
对24例原发性血小板增多症（ET）患者、20例反应性血小板增多症（RT）患者、20例真性红细胞增多症伴血小板增多症（PRVtr）患者、16例慢性粒细胞白血病伴血小板增多症（CMLtr）患者以及18名正常对照者（C）的骨髓巨核细胞祖细胞（BMsCFU-Mk）的自发集落形成情况进行了研究。体外甲基纤维素试验所用的培养基含有来自1例遗传性血色素沉着症患者的30%血浆。所有ET患者均记录到显著的BMsCFU-Mk生长，但RT患者和正常对照者均未出现。20例PRVtr患者中有11例、16例CMLtr患者中有7例存在BMs-CFU-Mk。还对这些患者的骨髓红系祖细胞（BMsBFU-E）进行了测定。24例ET患者中有21例发现BMsBFU-E，RT患者和正常对照者均未发现。所有PRVtr患者及1例CMLtr患者均显示有BMsBFU-E。我们得出结论，我们实施的体外甲基纤维素试验使得BMsCFU-Mk能够作为区分ET和RT的明确检测方法，这在以前发表的研究中尚未得到证实。此外，我们提供的证据表明，在10例患者中，试验中的BMsCFU-Mk和/或BMsBFU-E生长在长期血液学缓解后仍持续存在。
