血卟啉单甲醚对白色念珠菌的光动力灭活作用
Photodynamic inactivation of Candida albicans by hematoporphyrin monomethyl ether.
作者信息
Liu Chengcheng, Hu Min, Zeng Xiaoyan, Nair Sean P, Xu Jiru
机构信息
Department of Pathogenic Microbiology & Immunology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, 76 West Yanta Road, Xi'an 710061, PR China.
Key Laboratory of Environment & Genes Related to Diseases (Xi'an Jiaotong University), Ministry of Education of China, 76 West Yanta Road, Xi'an 710061, PR China.
出版信息
Future Microbiol. 2016;11(3):351-62. doi: 10.2217/fmb.15.142. Epub 2016 Mar 2.
AIM
To evaluate the capacity of hematoporphyrin monomethyl ether (HMME) in the presence of light to cause photodynamic inactivation (PDI) of Candida albicans.
MATERIALS & METHODS: HMME photoactivity was evaluated against azole-susceptible and -resistant C. albicans. The mechanisms by which PDI of C. albicans occurred were also investigated.
RESULTS
HMME-mediated PACT caused a dose-dependent inactivation of azole-susceptible and -resistant C. albicans. Incubation with 10 μM HMME and irradiation with 72 J cm(-2) light decreased the viability of C. albicans by 7 log10, induced damage of genomic DNA, led to loss of cellular proteins and damaged the cell wall, membrane and intracellular targets.
CONCLUSION
Candida albicans can be effectively inactivated by HMME in the presence of light, and HMME-mediated PACT shows its potential as an antifungal treatment.
目的
评估血卟啉单甲醚（HMME）在光照条件下对白色念珠菌进行光动力灭活（PDI）的能力。
材料与方法
评估HMME对唑类敏感和耐药的白色念珠菌的光活性。还研究了白色念珠菌发生PDI的机制。
结果
HMME介导的光活化化疗导致唑类敏感和耐药的白色念珠菌出现剂量依赖性失活。用10μM HMME孵育并照射72 J cm(-2) 的光，可使白色念珠菌的活力降低7个对数级，诱导基因组DNA损伤，导致细胞蛋白质丢失，并损伤细胞壁、细胞膜和细胞内靶点。
结论
在光照条件下，HMME可有效灭活白色念珠菌，且HMME介导的光活化化疗显示出作为抗真菌治疗方法的潜力。
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