Sandmann Rabea, Köster Sarah
Institute for X-Ray Physics, Georg-August-Universität Göttingen, Göttingen, 37077, Germany.
Sci Rep. 2016 Mar 3;6:22357. doi: 10.1038/srep22357.
Blood platelets are instrumental in blood clotting and are thus heavily involved in early wound closure. After adhering to a substrate they spread by forming protrusions like lamellipodia and filopodia. However, the interaction of these protrusions with the physical environment of platelets while spreading is not fully understood. Here we dynamically image platelets during this spreading process and compare their behavior on smooth and on structured substrates. In particular we analyze the temporal evolution of the spread area, the cell morphology and the dynamics of individual filopodia. Interestingly, the topographic cues enable us to distinguish two spreading mechanisms, one that is based on numerous persistent filopodia and one that rather involves lamellipodia. Filopodia-driven spreading coincides with a strong response of platelet morphology to the substrate topography during spreading, whereas lamellipodia-driven spreading does not. Thus, we quantify different degrees of filopodia formation in platelets and the influence of filopodia in spreading on structured substrates.
血小板在血液凝固过程中发挥着重要作用，因此在伤口早期愈合中也起着关键作用。血小板粘附到基质上后，会通过形成诸如片状伪足和丝状伪足等突起而展开。然而，这些突起在展开过程中与血小板物理环境之间的相互作用尚未完全明晰。在此，我们对血小板在展开过程进行动态成像，并比较它们在光滑和结构化基质上的行为。特别地，我们分析了展开面积随时间的变化、细胞形态以及单个丝状伪足的动态变化。有趣的是，地形线索使我们能够区分两种展开机制，一种基于大量持续存在的丝状伪足，另一种则更多地涉及片状伪足。由丝状伪足驱动的展开与展开过程中血小板形态对基质地形的强烈响应相吻合，而由片状伪足驱动的展开则不然。因此，我们量化了血小板中丝状伪足形成的不同程度以及丝状伪足在结构化基质上展开过程中的影响。
