Kara Murat, Kirkil Gamze, Kalemci Serdar
Department of Medical Genetics, Faculty of Medicine, Mugla Sitki Kocman University, Turkey.
Department of Chest Diseases, Faculty of Medicine, Firat University, Turkey.
Adv Clin Exp Med. 2016 Jan-Feb;25(1):21-6. doi: 10.17219/acem/28343.
This study aimed to investigate the associations of miRNA with COPD patients.
Chronic obstructive pulmonary disease (COPD) is characterized by progressive and largely irreversible airflow limitation. COPD is one of the most common causes of death globally and it is still a serious public health problem worldwide. Pathogenesis of COPD is multifactorial including genetics and environmental factors.
Sixty patients who were diagnosed with COPD according to GOLD guidance and 40 controls were involved in the study. This study was separated into four groups according to GOLD guidance. miR_16, miR_17, miR_29c, miR_92, miR_125, miR_126, miR_146, miR_155, miR_181, mir_122 expressions from the total miRNAs obtained were worked on by using real time-PCR method. The p-values are calculated based on a Student's t-test of the replicate 2^ (- Delta Ct) values for each gene in the control group.
The miRNAs expressions in normal and COPD patients were found differentially. The miR-29c (p = 0.043) and -126 (p = 0.012) were found significantly different compared to control group. When their expressions are evaluated according to stage, miR-92 expression showed down regulation stage II and no change was observed in other miRNAs. miR-29c and miR-126 expressions showed significant differences in stage III and only miR-126 expression showed significant difference in stage IV.
These results show that miRNA evaluations may give information about the diagnosis, staging and prognosis of the disease. In this study, we demonstrated that miR-29c and -126 are essential for the development of COPD.
本研究旨在调查微小RNA（miRNA）与慢性阻塞性肺疾病（COPD）患者之间的关联。
慢性阻塞性肺疾病（COPD）的特征是进行性且在很大程度上不可逆的气流受限。COPD是全球最常见的死亡原因之一，在世界范围内仍然是一个严重的公共卫生问题。COPD的发病机制是多因素的，包括遗传和环境因素。
根据慢性阻塞性肺疾病全球倡议（GOLD）指南诊断为COPD的60例患者和40例对照参与了本研究。本研究根据GOLD指南分为四组。使用实时聚合酶链反应（PCR）方法对所获得的总miRNA中的miR_16、miR_17、miR_29c、miR_92、miR_125、miR_126、miR_146、miR_155、miR_181、mir_122表达进行研究。基于对照组中每个基因的重复2^（-ΔCt）值的学生t检验计算p值。
发现正常人和COPD患者的miRNA表达存在差异。与对照组相比，miR-29c（p = 0.043）和-126（p = 0.012）有显著差异。当根据疾病阶段评估其表达时，miR-92表达在II期显示下调，其他miRNA未观察到变化。miR-29c和miR-126表达在III期有显著差异，仅miR-126表达在IV期有显著差异。
这些结果表明，miRNA评估可能为疾病的诊断、分期和预后提供信息。在本研究中，我们证明了miR-29c和-126对COPD的发展至关重要。
